橡胶树杂交F_(1)代抗病种质鉴选及其抗病性分析  

Selection and Analysis of Disease-Resistant Germplasms in Hybrid F_(1) Generations of Rubber Tree

作　　者：李博勋[1,2] 黄贵修 和丽岗[3] 蔡吉苗[1] 冯艳丽[1] 余文才 刘忠亮[3] LI Boxun;HUANG Guixiu;HE Ligang;CAI Jimiao;FENG Yani;YU Wencai;LIU Zhongiang(Environment and Plant Protection Institute,Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Integrated Pest Management on Tropical Grops,Ministry of Agriculture and Rural Affairs/Hainan Key Laboratory for Monitoring and Control of Tropical Agricultural Pests,Haikou,Hainan 571101,China;College of Plant Protection,Yunnan Agricultural University,Kun-ming,Yunnan 650201,China;Yunnan Institute of Tropical Crops,Jinghong,Yunnan 666100,China)

机构地区：[1]中国热带农业科学院环境与植物保护研究所/农业农村部热带作物有害生物综合治理重点实验室/海南省热带农业有害生物监测与控制重点实验室,海南海口571101 [2]云南农业大学植物保护学院,云南昆明650201 [3]云南省热带作物科学研究所,云南景洪666100

出　　处：《热带作物学报》2023年第11期2281-2291,共11页Chinese Journal of Tropical Crops

基　　金：海南省重点研发计划项目(No.ZDYF2021XDNY153);国家重点研发计划项目(No.2019YFD1000500)。

摘　　要：橡胶树棒孢霉落叶病(Corynespora leaf fall disease,CLFD)是全球主要植胶国最为严重的叶部病害之一,可造成严重的产量和经济损失,而抗病种质鉴选与创制利用是该病最为有效的防治策略。本研究对云研277-5×IAN 873、RRIC103×热研8-79和云研277-5×热垦525三个杂交组合的821份F_(1)代群体进行了抗棒孢霉落叶病的评价,明确了F_(1)代群体的抗病性水平,并从符合正态分布的2个杂交组合中筛选出32份候选F_(1)代单株进行芽接,再分别利用3个亚型的多主棒孢病菌和2种评价方法对候选F_(1)代无性系种苗进行抗病性复筛,最终获得5份抗病性较好的F_(1)代新种质。通过对5份抗病新种质防御酶活性的测定,以及抗病相关基因表达特性的分析,进一步证实了5份抗病新种质与多主棒孢病菌在侵染过程中的互作关系,明确了其在病原菌接种不同时间段的差异表达特征。本研究为橡胶树棒孢霉落叶病抗病性早期鉴选、抗病种质培育与创制利用提供了很好的种质材料和理论支撑。Corynespora leaf fall disease is one of the most severe leaf diseases in major rubber planting countries worldwide,which can cause serious yield and economic losses.The selection,creation,and utilization of resistant germplasms are the most effective prevention and control strategies for this disease.This study evaluated the disease resistance of 821 F_(1) populations from three hybrid combinations Yunyan 277-5×IAN 873,RRIC103×Reyan 8-79,Yunyan 277-5×Reken 525 and identified the level of disease resistance.Based on the evaluation results of disease resistance,32 candidate F_(1) generation individual plants were selected from two hybrid combinations that conform to normal distribution.Using three divergent clusters of Corynespora cassiicola and two evaluation methods to identify the resistant candidate F_(1) clone seedlings,five resistant germplasms were ultimately obtained by measuring the activity of defense enzymes and analyzing the expression characteristics of disease-related genes in five new resistant germplasms.Further investigation confirmed that the interaction relationship between five new resistant germplasms and multi host C.cassiicola during the infection process.This study provides excellent germplasm materials and a theoretical support for the early selection,cultivation,and utilization of resistant germplasms for the disease resistance of Corynespora leaf fall disease.

关 键 词：橡胶树 棒孢霉落叶病 杂交F_(1)代 鉴选 抗病性 

分 类 号：S794.1[农业科学—林木遗传育种]