台湾独蒜兰生长发育过程中源库关系转换研究  

Source-sink Exchange During the Growth and Development of Pleione formosana

作　　者：韩茹 游乐 江鸣涛 汪得凯 陈妮[1] 翟俊文[1] 吴沙沙[1] HAN Ru;YOU Le;JIANG Mingtao;WANG Dekai;CHEN Ni;ZHAI Junwen;WU Shasha(College of Landscape Architecture and Art,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China;Xiamen Municipal Works and Gardens Administration Bureau,Xiamen,Fujian 361001,China;College of Life Science and Medicine,Zhejiang Sci-Tech University,Hangzhou,Zhejiang 310018,China)

机构地区：[1]福建农林大学风景园林与艺术学院,福建福州350002 [2]福建省厦门市市政园林局,福建厦门361001 [3]浙江理工大学生命科学与医药学院,浙江杭州310018

出　　处：《热带作物学报》2023年第11期2330-2342,共13页Chinese Journal of Tropical Crops

基　　金：福建农林大学2021年度乡村振兴服务团队项目(No.11899170151);浙江省省级重点研发项目(No.2021C02043);国家林草局珍稀濒危物种调查监管与行业规范项目(No.2020073001)。

摘　　要：光合同化物具体的运输途径影响着营养物质在不同生长阶段的资源分配,最重要的是可以影响开花质量和果实品质,促进观赏植物的人工栽培和生产。本研究以台湾独蒜兰(Pleioneformosana)为研究对象,利用羧基荧光素(CFDA)示踪和激光扫描共聚焦显微技术,分别研究休眠期、盛花期、旺盛生长期和半枯期4个不同生长发育阶段的同化物运输方向,以了解不同器官在不同时期担负的源、库功能。结果发现:(1)在休眠期,同化物从母球导入经过鳞茎盘后最终运输到芽,以供新芽的生长和发育;(2)在盛花期,同化物主要从母球向花器官运输且运输方向不可逆,以保证开花的数量和质量;(3)在旺盛生长期,同化物主要从叶片输出供给叶结构部位和母球顶端新萌生顶芽球的生长;(4)在半枯期,2个新球之间竞争来自母球的营养物质,同时仍有一部分同化物用于满足顶芽球的生长发育。研究结果表明,台湾独蒜兰不同的营养器官或组织在不同生长发育阶段扮演的源、库角色各不相同,明确其在不同阶段发挥的具体功能,为今后的人工栽培和养护管理、商业化生产提供参考依据。The specific transport routes of photosynthetic compounds affect the resource allocation of nutrients at different growth stages.The most important is to affect the quality of flowering and fruit,and promote the artificial cultivation and production of ornamental plants.In this study,Pleione formosana,an endangered species,was used as the material,carboxylfluorescein(CFDA) tracer and laser scanning confocal microscopy were used to study the assimilate transport directions in four different growth and development periods,namely,dormancy period,blossom period,vigorous growth period and half dry period to understand the function of source and reservoir of different organs in different periods.During the dormancy period,assimilates were introduced from mother pseudobulb to bulb plate and finally transported to bud for the growth and development of new bud.In the blossom period,the assimilates were transported irreversibly from the mother bulb to the flower organs to ensure the quantity and quality of flowering.In the vigorous growth period,the assimilates mainly supplied the growth of the leaf and new bulbils at the apex of the mother pseudobulb.In the half dry period,the two daughter pseudobulbs competed with each other for nutrients from the mother pseudobulb,while some assimilates were still used to satisfy the growth and development of apex bulbils.The results showed that pseudobulb,as a nutrient storage organ,played an important role in assimilate distribution and generation regeneration succession in four different periods.Its special function would provide reference for artificial cultivation and commercial production in the future.

关 键 词：台湾独蒜兰 假鳞茎 羧基荧光素 源库转换 

分 类 号：S682.31[农业科学—观赏园艺]