棉花苗期耐盐相关性状QTL元分析  

Meta-analysis of QTL for Salt Tolerance-related Traits at Seeding Stage in Cotton

作　　者：杨文俊 朱雨婷 张杰[1] 徐凯祥 韦聪敏 陈全家[1] YANG Wenjun;ZHU Yuting;ZHANG Jie;XU Kaixiang;WEI Congmin;CHEN Quanjia(Xinjiang Key Laboratory of Crop Genetic Improvement and Germplasm Innovation,College of Agriculture,Xinjiang Agricultural University,Urumqi 830052,China)

机构地区：[1]新疆农业大学农学院,新疆作物遗传改良与种质创新重点实验室,乌鲁木齐830052

出　　处：《中国农业科技导报》2023年第12期26-34,共9页Journal of Agricultural Science and Technology

基　　金：新疆维吾尔自治区重大科技专项计划项目(2021A02001-3)。

摘　　要：为挖掘控制棉花苗期耐盐的重要数量性状位点(quantitative trait loci,QTL),利用BioMercator V4.2.3软件,以棉花高密度遗传连锁图谱作为参考,对来自3个作图群体涉及19个性状的194个QTLs进行图谱整合、映射以及QTL元分析。结果表明,通过建立棉花苗期耐盐相关性状一致性图谱,共挖掘出11个一致性QTL(meta quantitative trait loci,MQTL)位点,各MQTL至少包含3个原始QTLs,置信区间最短缩小至0.92 cM,分布于A03、A06、A11、A12、A13、D01、D03、D06、D07和D08共10条染色体上。通过对A11染色体上MQTL区间进行候选基因预测,挖掘到14个与棉花苗期耐盐相关的候选基因,为棉花苗期耐盐相关性状精细定位及分子辅助选择育种提供理论依据。To explore the real quantitative trait loci(QTL)controlling salt tolerance at seeding stage in cotton,194 QTLs for 19 traits from 3 mapping populations were integrated,mapped and QTL meta-analysis by BioMercator V4.2.3 software,which the high-density genetic linkage map of cotton was as a reference.The results showed that a total of 11 meta quantitative trait loci(MQTL)of salt tolerance-related traits at seeding stage were mined by the QTL consensus map in cotton,and each MQTL contained at least 3 original QTLs,which located on 10 chromosomes including A03,A06,A11,A12,A13,D01,D06,D07 and D08.The shortest confidence interval narrowed to 0.92 cM.Candidate gene prediction of MQTL intervals on chromosome A11 showed that 14 candidate genes were associated with salt tolerance at seeding stage in cotton.These results provided a theoretical basis for fine mapping of QTL for salt tolerance-related traits at seeding stage in cotton and molecular selection-assisted breeding.

关 键 词：棉花 耐盐 元分析 一致性QTL 候选基因 

分 类 号：S562[农业科学—作物学]