黑龙江省4个主栽水稻品种GS3基因克隆及表达模式分析  

Cloning and Expression Analysis of GS3 in Four Main Rice Varieties from Hei⁃longjiang Province

作　　者：张擘[1] 杨学文 赵北平[1] 郑福余[1] 孙中义[1] 孟庆英[3] 吴立成[1] 肖明纲[1] ZHANG Bo;YANG Xuewen;ZHAO Beiping;ZHENG Fuyu;SUN Zhongyi;MENG Qingying;WU Licheng;XIAO Minggang(Institute of Biotechnology,Heilongjiang Academy of Agricultural Sciences/Key Laboratory of Crop and Livestock Molecular Breeding of Heilongjiang Province,Harbin 150028;College of Biology and Agriculture,Jiamusi Univer-sity,Jiamusi 154007;Jiamusi Branch of Heilongjiang Academy of Agricultural Sciences,Jiamusi 154007,China)

机构地区：[1]黑龙江省农业科学院生物技术研究所/黑龙江省作物与家畜分子育种重点实验室,哈尔滨150028 [2]佳木斯大学生物与农业学院,黑龙江佳木斯154007 [3]黑龙江省农业科学院佳木斯分院,黑龙江佳木斯154007

出　　处：《东北农业科学》2023年第5期61-65,共5页Journal of Northeast Agricultural Sciences

基　　金：黑龙江省农业科学院资助项目(2018YYYF023、2020YYYF060);国家重点研发计划项目(2018YFD0200204-01)。

摘　　要：GS3是克隆的第一个负调控水稻粒长和粒重的基因。本研究在黑龙江省4个主栽水稻品种龙粳31、绥粳18、龙稻18和龙稻21中克隆GS3基因编码区并进行序列分析,发现4个品种的GS3基因都存在可变剪切,共检测到5种剪切类型。根据编码蛋白序列预测,有3种剪切类型是有功能类型。实时定量PCR检测结果表明,4个品种GS3基因均以有功能的剪切类型为主,在穗发育过程中表达呈现逐渐降低的趋势,长粒品种幼穗中表达量低于圆粒品种。本试验结果表明,这4个品种中GS3基因在穗发育过程中对粒长起负调控作用,可作为遗传操作靶位点进行粒形改良。GS3 is the first cloned gene that negatively regulates grain length and grain weight in rice.In this study,the coding region of GS3 in four main rice varieties(Longjing 31,Suijing 18,Longdao 18 and Longdao 21)from Hei⁃longjiang Province was cloned and sequenced.Alternative splicing of GS3 was detected in each of the four varieties and a total of five splicing patterns were identified,three of which were recognized as functional according to the amino acid sequence analysis on their coding protein.As was shown in real-time quantitative PCR results,func⁃tional splicing types were the most abundant splicing patterns dominant in the four varieties and their expression de⁃creased gradually during the panicle development.The expression varied significantly among the four varieties.GS3 expression level in long-seed varieties was lower than that in short-seed varieties.It was concluded that GS3 gene played vital roles in negative regulating seed length during panicle development in the four varieties,which could be used as the target gene for genetic operation to improve grain shape.

关 键 词：水稻 粒长 GS3 可变剪切 基因表达 

分 类 号：S511[农业科学—作物学] Q781[生物学—分子生物学]