吉林省甘薯双生病毒鉴定  

Identification of Geminiviruses in Sweet Potato Samples Collected from Jilin Province

作　　者：张春雨[1] 梁雨欣 李小宇[1] 刘建青 刘峰[1] 王永志[1] ZHANG Chunyu;LIANG Yuxin;LI Xiaoyu;LIU Jianqing;LIU Feng;WANG Yongzhi(Jilin Academy of Agricultural Sciences,Gongzhuling 136100;Jilin Agricultural University,Changchun 130118;Changchun Normal University,Changchun 130032,China)

机构地区：[1]吉林省农业科学院,吉林公主岭136100 [2]吉林农业大学,长春130118 [3]长春师范大学,长春130032

出　　处：《东北农业科学》2023年第5期66-70,共5页Journal of Northeast Agricultural Sciences

基　　金：吉林省农业科技创新工程项目(CXGC2021ZY022)。

摘　　要：本研究利用小RNA深度测序技术对吉林省7个甘薯产区采集的85份样本进行鉴定。发现混合样品中含有双生病毒科(Geminiviridae)菜豆金色花叶病毒属(Begomovirus)和玉米线条病毒属(Mastrevirus)的同源序列。经PCR扩增、测序鉴定,有16份样品感染玉米线条病毒属的甘薯无症状1号病毒Sweet potato symptomless virus(SPSMV1),21份样品感染菜豆金色花叶病毒属的甘薯曲叶病毒Sweet potato leaf curl virus(SPLCV)。In this study,we identified 85 samples of sweet potato leaf from 7 areas in Jilin Province by small RNA deep sequencing.The homologous sequences of virus in genus Begomovirus and Mastrevirus,family Geminiviridae were found from the total RNA.We identified 16 samples got SPSMV1 and 21 samples got SPLCV by PCR amplifi⁃cation and sequencing.

关 键 词：小RNA深度测序 双生病毒 甘薯无症状1号病毒 甘薯曲叶病毒 

分 类 号：S531.08[农业科学—作物学]