越橘氮素吸收利用的年周期变化规律研究  

Study on the Regularity of Annual Cycle Change of Nitrogen Uptake and Utili⁃zation in Blueberry

作　　者：唐雪东[1] 王思语 范家赫 蔡佳欣 高雪 谭锐阳 李亚东[1] TANG Xuedong;WANG Siyu;FAN Jiahe;CAI Jiaxin;GAO Xue;TAN Ruiyang;LI Yadong(College of Horticulture,Jilin Agricultural University,Changchun 130118,China)

机构地区：[1]吉林农业大学园艺学院,长春130118

出　　处：《东北农业科学》2023年第5期107-111,共5页Journal of Northeast Agricultural Sciences

基　　金：国家重点研发计划项目(2017YFC0504205);吉林省科技发展计划项目(20200702021NC)。

摘　　要：以越橘品种北陆为试材,分别于休眠期、萌芽期、幼果期、果实膨大期、果实成熟期、落叶期6个时期采集叶片、果实、新梢、一年生枝条、二年生枝条,对总氮含量、可溶性蛋白含量、谷丙转氨酶活性、谷氨酰胺合成酶活性进行测定。结果表明,在年周期内北陆越橘不同器官总氮含量为1.05%~2.98%,可溶性蛋白含量为0.62~2.82 mg/g,谷丙转氨酶活性为3.87~15.36μmol/(g·h)·FW,谷氨酰胺合成酶活性为0.32~2.99μmol/(g·h)·FW。年周期中越橘叶片和新梢总氮含量、可溶性蛋白含量、谷丙转氨酶活性、谷氨酰胺合成酶活性总体呈先上升后下降的趋势,枝条中总氮含量、可溶性蛋白含量、谷丙转氨酶活性、谷氨酰胺合成酶活性总体呈现先下降后上升的趋势,果实中总氮、可溶性蛋白含量呈现逐渐上升的趋势,谷丙转氨酶活性呈现先下降后上升的趋势,谷氨酰胺合成酶活性呈现先下降后上升再下降的趋势。在年周期内,北陆越橘树体氮素含量表现为叶片>果实>新梢>韧皮部>木质部。With the blueberry cultivar´Northland´as the test material,samples were taken at six stages,namely,dor⁃mancy stage,germination stage,young fruit stage,fruit expansion stage,fruit maturity stage and deciduous stage,re⁃spectively.Taking leaves,fruits,new shoots,annual branches and biennial branches,total nitrogen content,soluble protein content,glutamic-pyruvic transaminase and glutamine synthetase were determined.The results show that in the annual cycle,the total nitrogen content of´Northland´blueberry was 1.05%-2.98%,soluble protein content was 0.62-2.82 mg/g,the content of glutamic-pyruvic transaminase was 3.87-15.36μmol/(g·h)·FW,and the content of glutamine synthetase was 0.32-2.99μmol/(g·h)·FW.The total nitrogen content of leaves and shoots,the soluble protein content,the activities of glutamic-pyruvic transaminase and glutamine synthase in the annual cycle of"Northland"blueberry showed an overall trend of first increasing and then decreasing.The total nitrogen content,the soluble protein content,the activity of glutamic-pyruvic transaminase and glutamine synthetase in the branches decreased first and then increased,the total nitrogen content and the soluble protein content in fruits showed a trend of gradual increase,the activity of glutamic-pyruvic transaminase showed a trend of first decrease and then in⁃crease,and the activity of glutamine synthase showed a trend of first decrease,then increase and then decrease.Dur⁃ing the annual cycle,nitrogen changes to the leaf>fruit>new shoots>phloem>xylem.

关 键 词：越橘 氮素 年周期 代谢规律 

分 类 号：S663.9[农业科学—果树学]