骨肉瘤组织PBX1表达及其对患者预后的影响  

Expression of PBX1 in osteosarcoma tissues and its influence on prognosis

作　　者：白伟[1] 王斌[1] 郭甲瑞[1] 朱光谱 朱丽艳 BAI Wei;WANG Bin;GUO Jiarui;ZHU Guangpu;ZHU Liyan(Department of Orthopedics,the Second People's Hospital of Jiaozuo City,Jiaozuo 454000,Henan,China;Department of Oncology,the First Affiliated Hospital of Zhengzhou University,Zhengzhou 450001,Henan,China)

机构地区：[1]焦作市第二人民医院骨科,河南焦作454000 [2]郑州大学第一附属医院肿瘤内科,河南郑州450001

出　　处：《检验医学》2023年第10期926-929,共4页Laboratory Medicine

基　　金：河南省科技攻关计划项目(LHGJ20190173)。

摘　　要：目的 探讨骨肉瘤组织中前B淋巴细胞白血病转录因子(PBX1)基因和蛋白表达与患者临床病理参数的相关性及其对预后的影响。方法 选取2013年1月—2016年1月焦作市第二人民医院骨肉瘤患者85例,检测其骨肉瘤组织及正常骨组织(距肿瘤边缘>5 cm)中PBX1蛋白和mRNA的表达。收集所有患者的临床资料。从患者术后第1天开始随访,随访时间为5年。采用Kaplan-Meier生存曲线分析骨肉瘤患者的生存情况。采用Cox比例风险回归分析评估影响骨肉瘤患者预后的危险因素。结果 骨肉瘤组织PBX1蛋白阳性率和mRNA相对表达量均显著高于正常骨组织(P<0.001)。Enneking分期ⅡB~Ⅲ期和发生远隔转移的骨肉瘤患者肿瘤组织PBX1蛋白阳性率和mRNA相对表达量分别高于Enneking分期Ⅰ~ⅡA期和无远隔转移的骨肉瘤患者(P<0.05)。Kaplan-Meier生存曲线分析结果显示,PBX1蛋白阳性患者生存时间和5年生存率均低于PBX1蛋白阴性患者(P<0.001)。Cox比例风险回归分析结果显示,Enneking分期ⅡB~Ⅲ期、发生远隔转移和PBX1蛋白阳性是影响骨肉瘤患者生存期的危险因素[风险比(HR)值分别为2.861、2.244、2.361,95%可信区间(CI)分别为1.204~6.799、1.047~4.814、1.258~5.265]。结论 骨肉瘤组织PBX1蛋白阳性率和mRNA表达量显著升高,且与Enneking分期和远隔转移有关,或可作为骨肉瘤患者早期诊疗和预后评估潜在的生物标志物。Objective To investigate the correlation of the expressions of pre-B-cell leukemia transcription factor 1(PBX1)gene and protein in osteosarcoma tissues with clinicopathologic parameters of patients and its influence on prognosis.Methods A total of 85 patients with osteosarcoma from the Second People's Hospital of Jiaozuo City from January 2013 to January 2016 were enrolled.The expressions of PBX1 protein and mRNA in osteosarcoma tissues and normal bone tissues(>5 cm from tumor edges)were determined,and the clinical data of patients were collected.The patients were followed up on the first day after surgery and were followed up for 5 years.Kaplan-Meier survival curve was used to analyze the survival status of patients with osteosarcoma.Cox proportional hazard regression model was used to analyze the influencing factors for prognosis in patients with osteosarcoma.Results The positive rate of PBX1 protein and the relative expression of mRNA in osteosarcoma tissues were higher than those in normal bone tissues(P<0.001).The positive rate of PBX1 protein and the mRNA relative expression in tumor tissues of patients with Enneking stageⅡB-Ⅲand osteosarcoma with distant metastasis were higher than those in Enneking stageⅠ-ⅡA and osteosarcoma without distant metastasis(P<0.05).Kaplan-Meier survival curve analysis showed that the mean survival time and 5-year survival rate of patients with PBX1 protein positive were lower than those of patients with PBX1 protein negative(P<0.001).The results of Cox proportional hazard regression model showed that Enneking stageⅡB-Ⅲ,distant metastasis and PBX1 protein positive were risk factors affecting the survival of patients with osteosarcoma[hazard ratios(HR)were 2.861,2.244 and 2.361,95%confidence intervals(CI)were 1.204-6.799,1.047-4.814 and 1.258-5.265,respectively].Conclusions The protein positive rate and mRNA expression of PBX1 in osteosarcoma tissues are increased,and they are related to Enneking stage and distant metastasis,which may be used as potential biomarkers f

关 键 词：前B淋巴细胞白血病转录因子 骨肉瘤 Enneking分期 远隔转移 

分 类 号：R446.1[医药卫生—诊断学]