烟管头草的化学成分及其体外抗白血病活性研究  

Chemical constituents from Carpesium cernuum and their anti-leukemia activities in vitro

作　　者：冯树慧 晏晨[1] 张卫青 梁伟[1] 李艳梅[3] 韦学耐 饶青[3] 马四补 FENG Shuhui;YAN Chen;ZHANG Weiqing;LIANG Wei;LI Yanmei;WEI Xuenai;RAO Qing;MA Sibu(Department of Pharmacy,People’s Hospital of Anshun City,Anshun 561000,Guizhou,China;Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China;The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences,Guiyang 550014,China)

机构地区：[1]安顺市人民医院药剂科,贵州安顺561000 [2]贵州中医药大学,贵阳550025 [3]贵州省中国科学院天然产物化学重点实验室,贵阳550014

出　　处：《广西植物》2023年第11期2139-2148,共10页Guihaia

基　　金：贵州省自然科学基金(黔科合平台人才[2020]5008,黔科合支撑[2020]4Y203号,黔科合基础-ZK[2021]一般569,黔科合基础-ZK[2022]一般293);安顺市科技计划项目(安市科社[2021]19号);贵州省卫生健康委员会科学技术基金(gzwkj2021-446);贵州省科技计划项目(黔科合支撑[2022]一般189)。

摘　　要：为研究烟管头草的化学成分及其对白血病细胞的体外抑制作用,该文采用硅胶柱层析、凝胶柱层析、大孔吸附树脂等方法对烟管头草(Carpesium cernuum)乙酸乙酯部位进行分离纯化,并运用1 H NMR、13 C NMR和MS等波谱技术对化合物进行结构鉴定,采用MTT法测定化合物1-10对白血病细胞(K562、HEL)的体外抑制作用。结果表明:(1)从烟管头草乙酸乙酯部位共分离鉴定了11个化合物,分别为2,9-epoxy-5,9-dihydroxy-8-angeloyloxy-11-methoxymethyl-4(15)-germacraen-6,12-olide(1)、cardivin D(2)、cernuumolide I(3)、cernuumolide J(4)、8-hydroxy-9,10-diisobutyryloxythymol(5)、(2 E,6 Z,10 E,12 R)-7-[(acetyloxy)methyl]-3,11,15-trimethylhexadeca-2,6,10,14-tetraene-1,12-diol(6)、9,10-dihydroxyoctadecanoate(7)、1,6-dihydroxy-8-hydroxymethyl-anthraquinone(8)、emodin(9)、4-megastigmen-3,9-dione(10)、β-谷甾醇(11)。其中,化合物1为新化合物,化合物5、7-10均为首次从天名精属中分离得到,化合物2、5-10均为首次从烟管头草中分离得到。(2)活性测试结果表明化合物cardivin D(2)、cernuumolide I(3)和cernuumolide J(4)对白血病细胞具有较好的体外抑制作用,其中对K562细胞的IC 50值分别为(2.27±0.46)、(5.53±0.41)、(3.90±0.80)μmol·L^(-1),对HEL细胞的IC 50值分别为(1.84±0.14)、(2.36±0.90)、(2.31±1.17)μmol·L^(-1)。该研究结果丰富了烟管头草的化学成分,为抗白血病药物的研发提供了物质基础。In order to study the chemical constituents from Carpesium cernuum and their inhibitory effects on leukemia cells in vitro.The chemical constituents from ethyl acetate fraction of C.cernuum were isolated and purified by silica gel column chromatography,Sephadex LH-20 column chromatography and macroporous adsorption resin,and their structures were identified by means of various spectroscopic techniques such as 1 H NMR,13 C NMR and MS.The inhibitory effects of compounds 1-10 on leukemia cells(K562,HEL)in vitro were determined by MTT assay.The results were as follows:(1)Eleven compounds were isolated and identified as 2,9-epoxy-5,9-dihydroxy-8-angeloyloxy-11-methoxymethyl-4(15)-germacraen-6,12-olide(1),cardivin D(2),cernuumolide I(3),cernuumolide J(4),8-hydroxy-9,10-diisobutyryloxythymol(5),(2 E,6 Z,10 E,12 R)-7-[(acetyloxy)methyl]-3,11,15-trimethylhexadeca-2,6,10,14-tetraene-1,12-diol(6),9,10-dihydroxyoctadecanoate(7),1,6-dihydroxy-8-hydroxymethyl-anthraquinone(8),emodin(9),4-megastigmen-3,9-dione(10),β-sitosterol(11).Among them,Compound 1 was identified as a new compound,compounds 5,7-10 were isolated from Carpesium for the first time,compounds 2,5-10 were isolated from C.cernuum for the first time.(2)The results of activity test showed that cardivin D(2),cernuumolide I(3)and cernuumolide J(4)had good inhibitory effects on leukemia cells in vitro.The IC 50 of compounds 2-4 against K562 cells and HEL cells were(2.27±0.46),(5.53±0.41),(3.90±0.80)μmol·L^(-1)and(1.84±0.14),(2.36±0.90),(2.31±1.17)μmol·L^(-1),respectively.The study enriches the chemical constituents of C.cernuum,and provides a material basis for the development of anti-leukemia drugs.

关 键 词：烟管头草 化学成分 分离纯化 结构鉴定 白血病细胞 抑制作用 

分 类 号：Q946.91[生物学—植物学]