ALS抑制类除草剂对西瓜子叶外植体分化的研究  

Study on the differentiation of watermelon cotyledon explants by ALS inhibitor herbicides

作　　者：万丽丽 王转茸 汤谧 曾红霞 张学军[2,3] 张娜 任俭 孙玉宏 朱志坤 WAN Li-li;WANG Zhuan-rong;TANG Mi;ZENG Hong-xia;ZHANG Xue-jun;ZHANG Na;REN Jian;SUN Yu-hong;ZHU Zhi-kun(Wuhan Academy of Agricultural Sciences,Wuhan 430065,China;The Research Center of Hami-melon,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China;Hainan Sanya Crop Breeding Experimental Center,Xinjiang Academy of Agricultural Sciences,Sanya 572014,Hainan,China;Caidian District Bureau of Agriculture and Rural Affairs,Wuhan 430199,China)

机构地区：[1]武汉市农业科学院,武汉430065 [2]新疆农业科学院哈密瓜研究中心,乌鲁木齐830091 [3]新疆农业科学院海南三亚农作物育种试验中心,海南三亚572014 [4]武汉市蔡甸区农业农村局,武汉430199

出　　处：《湖北农业科学》2023年第11期202-206,213,共6页Hubei Agricultural Sciences

基　　金：湖北省重点研发项目(2022BBA0062,2021BBA101);天山创新团队计划项目(2022D14015);武汉市生物育种关键技术及新品种培育重大专项(2022021302024852);武汉市农业科学院创新项目(XKCX202204-1)。

摘　　要：以西瓜自交系D66的子叶为外植体,将外植体置于含有不同浓度乙酰乳酸合成酶(Acetolactate synthase,ALS)抑制剂类除草剂(SU除草剂、IMI除草剂)的筛选培养基上,根据外植体的分化率确定最适宜的除草剂浓度。探讨不同植物生长调节剂(6-BA、NAA)浓度配比对不定芽的增殖作用。结果表明,SU除草剂中苯磺隆和噻吩磺隆的最适宜浓度均为0.25 mg/L,苄嘧磺隆的最适宜浓度为0.50 mg/L;IMI除草剂中灭草喹和灭草烟的最适宜浓度均为1.50 mg/L;在MS+1.0 mg/L 6-BA+0.5 mg/L NAA培养基中不定芽的增殖率最高,为287.0%;根据西瓜自交系材料D66确定的除草剂最适宜浓度同样适用于西瓜杂交种(武农8号、黑美人、甜王1号、早佳8424)子叶外植体的筛选试验。Using the cotyledons of watermelon inbred line D66 as explants,the explants were placed on a screening medium containing different concentrations of acetolactate synthase(ALS)inhibitor herbicides(SU herbicides,IMI herbicides),and the optimal herbi⁃cide concentration was determined based on the differentiation rate of the explants.The effect of different plant growth regulators(6-BA,NAA)concentration ratios on the proliferation of adventitious buds was explored.The results showed that the optimal concentra⁃tions of tribenuron-methy1 and thifensulfuron-methy1 in SU herbicides were both 0.25 mg/L,while the optimal concentration of ben⁃sulfuron was 0.50 mg/L;the most suitable concentrations for IMI herbicides such imazaquin and imazapyr were both 1.50 mg/L;the highest proliferation rate of adventitious buds was observed in MS+1.0 mg/L 6-BA+0.5 mg/L NAA medium,which was 287.0%;the op⁃timal concentration of herbicides determined based on watermelon inbred line D66 materials was also suitable for screening cotyledon explants of watermelon hybrids(Wunong 8,Black Beauty,Tianwang 1,Zaojia 8424).

关 键 词：子叶外植体 ALS抑制类除草剂 西瓜自交系 西瓜杂交种 不定芽增殖 SU除草剂 IMI除草剂 

分 类 号：S651[农业科学—果树学]