CFS预处理对不同秸秆原料酶解和理化结构的影响  

Effects of CFS Pretreatment on Enzymatic Hydrolysis and Physicochemical Structure of Different Straw Materials

作　　者：田鑫 王雨萌 徐师苗 汪强杰 胡轲 张海波 程红艳[1] TIAN Xin;WANG Yumeng;XU Shimiao;WANG Qiangjie;HU Ke;ZHANG Haibo;CHENG Hongyan(College of Resources and Environment,Shanxi Agricultural University,Taigu 030801,China)

机构地区：[1]山西农业大学资源环境学院,山西太谷030801

出　　处：《山西农业科学》2023年第12期1426-1434,共9页Journal of Shanxi Agricultural Sciences

基　　金：山西省高等学校科技创新项目(2020L0137);山西农业大学科技创新基金项目(2018YJ39);山西省优秀博士来晋工作奖励基金(SXYBKY201803);国家自然科学基金(52100149);山西省水利科学技术研究与推广项目(2022GM034)。

摘　　要：高铁酸钾复合液(CFS)是制备高铁酸钾的剩余滤液,其含有大量碱(OH^(-))和氧化剂(ClO^(-)和Fe^(6+)),具有破坏木质纤维素顽固结构、提升酶解效率的潜力。为实现秸秆的资源化利用与高铁酸钾制备废液的再利用,以山西储量丰富的玉米秸秆(CS)、高粱秸秆(SS)和谷子秸秆(MS)为原料,采用CFS进行预处理,对比3种秸秆的酶解糖化率,分析秸秆的理化结构变化。结果表明,CFS预处理中碱和氧化剂共同参与了3种秸秆的降解,促进了酶解糖化率;在最佳预处理时间24 h下,CS、SS和MS的还原糖产量分别较对照提高252.77%、236.39%、216.66%,其中CS的酶解效率最高;组分分析表明,CFS处理能有效去除3种秸秆中木质素成分,增加纤维素相对含量,进而有利于纤维素酶的可及性;结构分析显示,CFS处理后,3种秸秆的理化结构发生了不同程度变化,粗糙度增加,官能团发生断裂,纤维结晶度升高,热稳定性变差。在3种秸秆中,CS结构变化最明显,更有利于被生物转化。综上,CFS预处理可改变作物秸秆的理化结构,破坏其致密结构,促进后续酶解效率,是一种理想的预处理技术。Composite ferrate solution(CFS)is the residual filtrate for preparing potassium ferrate.It contains a lot of alkali(OH^(-))and oxidant(ClO^(-)and Fe^(6+)),which has the potential to destroy the recalcitrant structure of lignocellulose and improve the efficiency of enzymatic hydrolysis.In order to realize the utilization of straw resources and reuse of preparation waste liquid of potassium ferrate,in this paper,corn straw(CS),sorghum straw(SS),and millet straw(MS),which are abundant in Shanxi province,were pretreated with CFS,the enzymolysis and saccharification rates of the three kinds of straw were compared,and the change of physicochemical structure of the straw was analyzed.The results showed that the alkali and oxidant in the pretreatment of CFS were involved in the degradation of three kinds of straw,which promoted the enzymatic hydrolysis rate and saccharification rate.Under the optimal pretreatment time of 24 h,the reducing sugar yield of CS,SS,and MS was increased by 252.77%,236.39%,and 216.66%compared with that of the control,respectively,and the enzymatic hydrolysis efficiency of CS was the highest.Component analysis showed that CFS treatment could effectively remove lignin in three kinds of straw and increase the relative content of cellulose,which was beneficial to the accessibility of cellulase.Structural analysis showed that after CFS treatment,the physicochemical structure of the three kinds of straw changed in different degrees,roughness increased,functional group fractured,fiber crystallinity increased,and thermal stability decreased.Among the three kinds of straw,CS had the most obvious structural change and was more conducive to biotransformation.In conclusion,CFS pretreatment could change the physicochemical structure of crop straws,destroy the dense structure and promote the efficiency of subsequent enzymatic hydrolysis,so it was an ideal pretreatment technology.

关 键 词：高铁酸钾复合液(CFS) 预处理 作物秸秆 还原糖产量 理化结构 

分 类 号：S141.4[农业科学—肥料学]