苗药吉祥草配伍余甘子抗Lewis肺癌细胞增殖组分的筛选  

Screening of Anti-Lewis Lung Cancer Cells Proliferation Components of Miao Medicine Reineckia Carnea Combined with Phyllanthus Emblica

作　　者：黄兴文 刘炜 HUANG Xingwen;LIU Wei(Guangdong Yunfu Vocational College of Traditional Chinese Medicine,Yunfu Guangdong 527400,China;The Second Affiliated Hospital of Guizhou University of Traditional Chinese Medicine,Guiyang Guizhou 550001,China)

机构地区：[1]广东云浮中医药职业学院,广东云浮527400 [2]贵州中医药大学第二附属医院,贵州贵阳550001

出　　处：《中医药导报》2023年第11期6-10,共5页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(82160952)。

摘　　要：目的:探索吉祥草和余甘子配伍后治疗非小细胞肺癌的药效物质,为吉祥草配伍余甘子在抗肿瘤制剂开发方面的研究提供实验依据。方法:采用鲜药榨汁、传统水煎、渗漉为提取方法制得吉祥草配伍余甘子的不同提取物,以Lewis肺癌细胞体外实验模型作为评价手段,优选药材的最佳提取方法;通过膜分离技术,将最佳提取方法的提取物分段截留得到不同分子量区间段组分,以Lewis肺癌细胞体外实验模型作为评价手段优选最佳的分子量区间段。结果:吉祥草配伍余甘子对Lewis肺癌细胞增殖抑制作用的最佳提取方法为95%乙醇渗漉法。95%乙醇渗漉提取物对Lewis肺癌细胞增殖抑制作用的最佳分子量区间段组分为分子量10~30 KD与分子量≤1 KD组分。结论:苗药吉祥草配伍余甘子对Lewis肺癌细胞具有增殖抑制作用,抑制作用最佳组分为95%乙醇渗漉的分子量10~30 KD与分子量≤1 KD组分。Objective:To explore the effective material of Miao medicine Reineckia carnea combined with Phyllanthus emblica for the treatment of non-small cell lung cancer in order to provide further experimental evidence for the research of the development of anti-tumor agents.Methods:Fresh juice,traditional medicine decoction,and percolation extraction method prepared different extracts of Reineckea carnea compatibility of Phyllanthus emblica.The best extraction method of medicinal materials was selected by using Lewis lung cancer cell in vitro experimental model as evaluation method.The extracts of the best extraction method were segmented to obtain different molecular weight interval components through the membrane separation technology.Then in vitro experimental model of Lewis lung cancer cells was applied to obtain the optimal molecular weight interval.Results:The best extraction method of Reineckia carnea compatibility with Phyllanthus emblica is 95%ethanol osmoticization.The best molecular weight interval components of 95%ethanol percolation extract are 10-30 KD and≤1 KD components.Conclusion:Miao medicine Reineckia carnea combined with Phyllanthus emblica can inhibit the proliferation of Lewis lung cancer cells.And the optimal components for inhibitory effect are components with a molecular weight of 10-30 KD and a molecular weight of≤1 KD,which are permeated with 95%ethanol.

关 键 词：肺癌 LEWIS肺癌细胞 吉祥草 余甘子 渗漉 膜分离技术 苗药 

分 类 号：R291.608[医药卫生—民族医学]