黄连丸剂、黄连汤剂及效应物小檗碱对2型糖尿病大鼠糖脂代谢的影响  

The Effects of Huanglian Pill(黄连丸剂),Huanglian Decoction(黄连汤剂)and Effector Substance Berberine on Glucose and Lipid Metabolism in Rats with Type 2 Diabetes

作　　者：姜淑君 邹欣[1] 董慧[1] 李井彬 周俪姗[2] 陆付耳[1] 魏世超 徐丽君[1] JIANG Shujun;ZOU Xin;DONG Hui;LI Jingbin;ZHOU Lishan;LU Fu'er;WEI Shichao;XU Lijun(Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan Hubei 430030,China;Wuhan Children's Hospital/Wuhan Maternal and Child Healthcare Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan Hubei 430016,China)

机构地区：[1]华中科技大学同济医学院附属同济医院,湖北武汉430030 [2]华中科技大学同济医学院附属武汉儿童医院/武汉市妇幼保健院,湖北武汉430016

出　　处：《中医药导报》2023年第11期11-17,共7页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(81473448)。

摘　　要：目的:观察黄连丸剂、黄连汤剂和小檗碱对糖尿病大鼠糖脂代谢的影响。方法:随机取6只大鼠作为对照组,40只大鼠给予高脂饮食结合尾静脉注射链脲佐菌素(STZ)建立糖尿病大鼠模型。将造模成功的30只糖尿病大鼠按照随机数字表法分为模型组、小檗碱高剂量组、小檗碱低剂量组、黄连丸剂组、黄连汤剂组,每组6只。小檗碱高、低剂量组大鼠分别予高剂量[156 mg/(kg·d)]、低剂量[78 mg/(kg·d)]小檗碱混悬液灌胃;黄连丸剂组大鼠予黄连粉末混悬液灌胃[0.64 mL/(kg·d)];黄连汤剂组大鼠予黄连煎剂浓缩液灌胃[0.62 mL/(kg·d)]。给药后第0、9、18、27、36、45、54、63天采集大鼠眼眶血,检测空腹血糖(FBG)和空腹胰岛素(FINS),计算胰岛素敏感性指标(ISI)。使用试剂盒检测第63天口服糖耐量试验(OGTT)及血脂水平。结果:高效液相色谱(HPLC)图显示黄连丸剂和黄连汤剂中均含有小檗碱成分。黄连丸剂和黄连汤剂在模拟胃液中小檗碱的质量分数分别为66.8 mg/g和38.3 mg/g。黄连丸剂组、黄连汤剂组和小檗碱高剂量组大鼠OGTT、FBG、FINS和血脂水平均有不同程度改善(P<0.05),而小檗碱低剂量组大鼠的OGTT、FBG、FINS和血脂水平无明显改善(P>0.05)。与黄连汤剂组和小檗碱高剂量组比较,黄连丸剂组大鼠OGTT、FBG、FINS和ISI改善更为明显(P<0.05),而各给药组大鼠血脂水平比较,差异无统计学意义(P>0.05)。从第45天开始,黄连丸剂组大鼠FBG水平改善效果优于黄连汤剂组和小檗碱高剂量组(P<0.05)。从第63天开始,黄连丸剂组大鼠FINS水平低于黄连汤剂组和小檗碱高剂量组(P<0.05)。黄连丸剂组大鼠ISI水平从第45天开始高于黄连汤剂组(P<0.05),从第54天开始高于小檗碱高剂量组(P<0.05)。结论:黄连丸剂、黄连汤剂和高剂量小檗碱对糖尿病大鼠血糖、胰岛素敏感性和血脂均有一定程度的改善作用。黄连丸剂在降血糖和改善�Objective:To observe the effects of Huanglian pill(HL-P),Huanglian decoction(HL-D)and berberine on glucose and lipid metabolism of diabetic rats.Methods:Totally six rats were randomly selected as control group,and 40 rats were given high fat diet and injected with streptozotocin(STZ)into the tail vein to establish diabetic rat model.Totally 30 diabetic rats were successfully modeled and randomly divided into model group,Berberine high dose group,Berberine low dose group,HL-P group and HL-D group,with six rats in each group.Berberine high dose and low dose groups were given high dose[156 mg/(kg·d)]and low dose[78 mg/(kg·d)]berberine suspension by gavage respectively.The rats in HL-P group were given Coptis powder suspension[0.64 mL/(kg·d)]by gavage.The rats in HL-D group were given Coptis decoction concentrate[0.62 mL/(kg·d)]by intragastric administration.Orbital blood was collected at 0,9,18,27,36,45,54 and 63 days after administration,then fasting blood glucose(FBG)and fasting insulin(FINS)were detected.And insulin sensitivity index(ISI)was calculated.Oral glucose tolerance test(OGTT)and blood lipid levels were measured by the kit on the 63rd day.Results:HPLC showed berberine is found in Coptis pills and Coptis decoction.The content of berberine in the simulated gastric fluid of Coptis pills and Coptis decoction was respectively 66.8 mg/g and 38.3 mg/g.The OGTT,FBG,FINS and blood lipid levels of diabetic rats were improved in HL-P group,HL-D group and berberine high-dose group(P<0.05),but the OGTT,FBG,FINS and blood lipid levels of diabetic rats were not improved in berberine low-dose group(P>0.05).Compared with HL-D group and berberine high-dose group,OGTT,FBG,FINS and ISI were improved more significantly in HL-P group at the end of treatment(P<0.05),while there was no significant difference in the improvement of blood lipid levels in diabetic rats among treatment groups(P>0.05).In addition,from the 45th day,the HL-P group showed better improvement of FBG level than HL-D group and berberine high-dose group(

关 键 词：2型糖尿病 黄连丸剂 黄连汤剂 小檗碱 血糖 血脂 大鼠 

分 类 号：R285.5[医药卫生—中药学]