“大推天河水”不同操作时长对发热幼兔的退热效果及下丘脑5-HT、NE的影响  

Antipyretic Effect of Different Operation Duration of"Large Tui-Pushing Tianheshui"(大推天河水)on Young Rabbits with Fever and the Effects on 5-HT and NE in Hypothalamus

作　　者：王慧娟 陈碧婵 龙倩 欧玲林 钟欢[2] 刘迈兰[2] 叶勇[1] 汤伟[1] WANG Huijuan;CHEN Bichan;LONG Qian;OU Linglin;ZHONG Huan;LIU Mailan;YE Yong;TANG Wei(The First Hospital of Hunan University of Chinese medicine,Changsha Hunan 410007,China;College of Acupuncture-Moxibustion and Tuina,Hunan University of Chinese Medicine,Changsha Hunan 410208,China)

机构地区：[1]湖南中医药大学第一附属医院,湖南长沙410007 [2]湖南中医药大学针灸推拿学院,湖南长沙410208

出　　处：《中医药导报》2023年第11期18-23,共6页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：湖南省自然科学基金(2021JJ70108);湖南省“十四五”第一批中医药领军人才和学科带头人培养项目(青年神农学者)(湘中医药[2022]4号);湖南省卫生健康高层次人才医学学科青年骨干人才培养对象(湘卫函[2023]78号)。

摘　　要：目的:观察“大推天河水”不同操作时长对内毒素致热幼兔的退热效果及下丘脑中5-羟色胺(5-HT)、去甲肾上腺素(NE)的影响,探究推拿操作时长与退热疗效的效应关系。方法:将40只新西兰幼兔随机分为正常组、模型组、大推天河水5 min组、大推天河水10 min组和布洛芬组,每组8只。模型组、大推天河水5 min组、大推天河水10 min组和布洛芬组通过耳缘静脉注射大肠杆菌内毒素(LPS,200 ng/mL),1 mL/kg,建立发热模型。大推天河水5 min组在造模后1.5 h于幼兔左前肢掌面正中行“大推天河水”手法,时长为5 min,频率为50~100次/min;大推天河水10 min组分别在造模后1.5、2.5 h行“大推天河水”手法,每次时长为5 min,累计时长为10 min,频率为50~100次/min;布洛芬组在造模后1.5 h予布洛芬混悬液(0.01 g/mL)灌胃,1 mL/kg。观测幼兔造模后体温动态变化,比较各组幼兔体温降至正常范围的时间,并应用酶联免疫吸附法(ELISA)测定下丘脑中5-HT及NE的含量。结果:大推天河水5 min组幼兔造模后2.0~3.0 h的体温上升高度明显低于模型组(P<0.01);大推天河水10 min组幼兔造模后1.5~3.5 h的体温上升高度低于模型组(P<0.05);布洛芬组幼兔造模后2.0~3.5 h、5.0~5.5 h的体温上升高度低于模型组(P<0.01);大推天河水10 min组幼兔在造模后2.5~4.0 h的体温上升高度明显低于大推天河水5 min组(P<0.01);布洛芬组幼兔在造模后3.0~4.0 h、5.5~6.0 h的体温上升高度低于大推天河水5 min组(P<0.05);布洛芬组幼兔造模后5.5~6.0 h的体温上升高度低于大推天河水10 min组(P<0.05)。大推天河水10 min组及布洛芬组幼兔退热时间明显短于模型组及大推天河水5 min组(P<0.01)。模型组幼兔下丘脑中5-HT含量低于正常组(P<0.01),NE含量高于正常组(P<0.01);大推天河水10 min组及布洛芬组幼兔下丘脑中5-HT含量高于模型组(P<0.01),NE含量低于模型组(P<0.01);大推天河水5 min组幼兔下丘脑�Objective:To observe the antipyretic effect of different operation duration of"Large Tui-pushing Tianheshui"on endotoxin-induced fever of young rabbits and the influence of 5-HT and NE neurotransmitters in hypothalamus,and explore the effect relationship between massage operation duration and antipyretic effect.Methods:Totally 40 New Zealand young rabbits were randomly divided into normal group,model group,Large Tui-pushing Tianheshui 5 min group,Large Tui-pushing Tianheshui 10 min group and an ibuprofen group,with 8 rabbits in each group.Rabbits in the model group,Large Tui-pushing Tianheshui 5 min group,Large Tui-pushing Tianheshui 10 min group and ibuprofen group were subjected to prepare the endotoxin-induced fever model through the intravenous lipopolysaccharide(200 ng/mL)from the ear marginal vein,1 mL/kg."Large Tui-pushing Tianheshui"manipulation was performed on the middle of the left forelimb of the young rabbits 1.5 h after modeling in the Large Tui-pushing Tianheshui 5 min group,for 5 minutes with a frequency of 50-100 times/min."Large Tui-pushing Tianheshui"manipulation was performed on the middle of the left forelimb of the young rabbits 1.5 and 2.5 h after modeling in Large Tui-pushing Tianheshui 10 min group.The duration of each session was 5 minutes,and the cumulative duration was 10 minutes.The frequency was 50-100 times/min.Ibuprofen group was given ibuprofen suspension(0.01 g/mL,1 mL/kg)by gavage at 1.5 h after modeling.The dynamic changes of body temperature of young rabbits after modeling were observed,and the time point when body temperature dropped to normal was compared.The contents of 5-HT and NE in hypothalamus were determined by enzyme-linked immunoadsorption(ELISA).Results:The height of body temperature rise in Large Tui-pushing Tianheshui 5 min group was significantly lower than that in model group at 2.0-3.0 h after modeling(P<0.01).The height of body temperature rise in Large Tui-pushing Tianheshui 10 min group was lower than that of the model group at 1.5-3.5 h after modeling(P<0.0

关 键 词：发热 大推天河水 小儿推拿 时间量效 5-羟色胺 去甲肾上腺素 兔 

分 类 号：R285.5[医药卫生—中药学]