水牛角对血热出血模型大鼠的止血作用及机制  

Hemostatic Effect and Mechanism of Cornu Bubali on Hemorrhage Model of Blood Heat Syndrome in Rats

作　　者：李海霞 周民 周有君 常依 王雅露 LI Haixia;ZHOU Min;ZHOU Youjun;CHANG Yi;WANG Yalu(Hunan Children's Hospital,Changsha Hunan 410007,China)

机构地区：[1]湖南省儿童医院,湖南长沙410007

出　　处：《中医药导报》2023年第11期30-34,共5页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：湖南省自然科学青年基金项目(2020JJ5280);湖南省卫生健康委科研项目(20200672);湖南省临床医疗技术创新引导项目(2021SK50514)。

摘　　要：目的:探讨水牛角对血热出血模型大鼠的止血作用及机制。方法:将60只SD大鼠随机分为正常对照组、出血模型组、云南白药组、水牛角高剂量组、水牛角中剂量组、水牛角低剂量组,每组10只。除正常对照组外,其余各组大鼠均采用干姜水煎液结合5%乙醇给药复制血热出血模型。造模6 h后,云南白药组大鼠予云南白药溶液(0.5 g/kg)灌胃,水牛角高、中、低剂量组大鼠分别灌胃高(36 g/kg)、中(18 g/kg)、低(9 g/kg)剂量水牛角水煎液,正常对照组、出血模型组大鼠灌胃等体积生理盐水。1次/d,连续14 d。末次给药1 h后检测各组大鼠体质量、饮水量、摄食量、尿量、粪便含水量、体质量增长率、肛温;腹主动脉取血检测凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)、纤维蛋白原(FIB)、凝血酶时间(TT)、纤维蛋白降解产物(FDP)、抗凝血酶(AT);HE染色观察肺、胃组织病理学变化。结果:出血模型组大鼠肛温高于正常对照组(P<0.01),体质量增长率、摄食量、饮水量、尿量、粪便量及粪便含水量均低于正常对照组(P<0.01);云南白药组大鼠肛温低于出血模型组(P<0.05),体质量增长率高于出血模型组(P<0.01);水牛角高、中剂量组大鼠肛温低于出血模型组(P<0.01或P<0.05),体质量增长率、摄食量、饮水量、尿量、粪便量及粪便含水量均高于出血模型组(P<0.01或P<0.05)。出血模型组大鼠PT、APTT均低于正常对照组,FIB、TT均高于正常对照组,差异均有统计学意义(P<0.01或P<0.05);云南白药组大鼠TT、FIB均低于出血模型组(P<0.05);水牛角高、中剂量组大鼠PT、APTT均高于出血模型组,FIB、TT均低于出血模型组,差异均有统计学意义(P<0.01或P<0.05)。出血模型组大鼠AT、FDP高于正常对照组(P<0.01);云南白药组大鼠FDP低于出血模型组(P<0.01);水牛角高、中剂量组大鼠AT、FDP均低于出血模型组(P<0.01)。正常对照组大鼠肺、胃组织�Objective:To investigate the hemostatic effect and mechanism of Cornu Bubali on hemorrhage model of blood heat syndrome in rats.Methods:Totally 60 SD rats were randomly divided into normal control group,hemorrhage model group,Yunnan Baiyao(云南白药)group,Cornu Bubali high-dose group,Cornu Bubali medium-dose group and Cornu Bubali low-dose group,with 10 rats in each group.Except the normal control group,the other groups were given the dried ginger decoction combined with 5%ethanol to create hemorrhage model of blood heat syndrome.After 6 hours of modeling,rats were given Yunnan Baiyao solution(0.5 g/kg)by gavage in Yunnan Baiyao group,and high(36 g/kg),medium(18 g/kg)and low dose(9 g/kg)Cornu Bubali decoction respectively in Cornu Bubali high-dose,medium-dose and low-dose groups.Normal control group and hemorrhage model group were given equal volume of normal saline,once a day for 14 consecutive days.Body mass,water intake,food intake,urine volume,fecal water content,body mass growth rate and anal temperature were measured 1 hour after the last administration.Prothrombin time(PT),activated partial thromboplastin time(APTT),fibrinogen(FIB),thrombin time(TT),fibrin degradation product(FDP)and antithrombin(AT)were detected.The histopathological changes of lung and stomach were observed by HE staining.Results:Hemorrhage model group showed higher anal temperature than normal control group(P<0.01),while lower growth rate of body mass,food intake,water intake,urine volume,fecal volume and fecal water content than those normal control group(P<0.01).Yunnan Baiyao group showed lower anal temperature than hemorrhage model group(P<0.05),while higher growth rate of body mass than hemorrhage model group(P<0.01).Cornu Bubali high-dose and medium-dose groups showed lower anal temperature than hemorrhage model group(P<0.01 or P<0.05),while higher body mass growth rate,food intake,water intake,urine volume,fecal volume and fecal water content than hemorrhage model group(P<0.01 or P<0.05).Hemorrhage model group showed lower PT a

关 键 词：血热出血 水牛角 凝血功能 大鼠 

分 类 号：R285.5[医药卫生—中药学]