基于JAK2/STAT3信号通路探究象皮生肌膏对肛瘘术后模型大鼠创面修复的影响  

Effect of Xiangpi Shengji Ointment(象皮生肌膏)on Wound Repair in Post-Operative Anal Fistula Model Rats Based on JAK2/STAT3 Signaling Pathway

作　　者：黄静雯 尹园缘 刘颖 邹巍莹 程扬 詹敏[1] 余炼[2] 宾东华[1,2] HUANG Jingwen;YIN Yuanyuan;LIU Ying;ZOU Weiying;CHENG Yang;ZHAN Min;YU Lian;BIN Donghua(The First Affiliated Hospital of Hunan University of Chinese Medicine,Changsha Hunan 410007,China;Hunan University of Chinese Medicine,Changsha Hunan 410208,China)

机构地区：[1]湖南中医药大学第一附属医院,湖南长沙410007 [2]湖南中医药大学,湖南长沙410208

出　　处：《中医药导报》2023年第11期55-61,共7页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：湖南省自然科学基金项目(2023JJ60339);湖南省临床医疗技术创新引导项目(2021SK51416);湖南省中医药管理局重点课题(C2022019);长沙市自然科学基金项目(Kq2202458);湖南中医药大学校级课题重点项目(2019XJJJ034);湖南中医药大学中西医结合一流学科开放课题重点项目(2020ZXYJH05);湖南中医药大学中西医结合一流学科开放课题一般项目(2020ZXYJH57);湖南中医药大学研究生创新课题一般项目(2022CX140,2022CX161)。

摘　　要：目的:基于Janus激酶白2(JAK2)/信号传导及转录激活因子3(STAT3)信号通路,探讨象皮生肌膏对肛瘘术后模型大鼠创面修复的影响。方法:将36只SD大鼠随机分为象皮生肌膏组、凡士林组、假手术组和模型组,每组9只。采用钢丝引流的方法对大鼠进行肛瘘疾病的造模后,实施肛瘘瘘管切开术(假手术组除外)建立大鼠肛瘘术后创面模型。模型组采用生理盐水治疗,象皮生肌膏组、凡士林组分别采用相应的药物治疗。各组予相应药物干预10 d,每组大鼠分别在给药第5、10天采用常规面积检测法检测各组大鼠对不同时间点的肛瘘术后创面面积;采用苏木素-伊红(HE)染色法观察各组大鼠给药第3、5、7、10天创面肉芽组织病理学情况;逆转录-聚合酶链反应(RT-qPCR)法测定肉芽组织中白介素-6(IL-6)mRNA、JAK2 mRNA、STAT3 mRNA相对表达量;Western blotting法检测各组大鼠给药第5、10天创面肉芽组织中IL-6、糖蛋白130(gp130)、JAK2、STAT3蛋白相对表达量。结果:给药第3天,3组大鼠创面面积较大,无红肿,有少量分泌物渗出。给药第5、7、10天,象皮生肌膏组创面面积缩小明显,模型组及凡士林组大鼠创面减少面积小于同时间点象皮生肌膏组。给药第10天,象皮生肌膏组大鼠大部分创面愈合,凡士林组和模型组大鼠创面未完全愈合。给药第5、7、10天,象皮生肌膏组、凡士林组大鼠创面愈合率高于模型组(P<0.01),象皮生肌膏组大鼠创面愈合率高于凡士林组(P<0.01)。HE染色结果显示,象皮生肌膏组大鼠创面基本修复,成纤维细胞致密排列,少量炎症细胞浸润;凡士林组大鼠创面受损修复较差,血管有扩张出血,较多炎症细胞浸润;模型组大鼠创面表层出现明显出血及坏死,组织高度水肿,伴大量炎症细胞浸润。象皮生肌膏组、凡士林组、模型组大鼠创面组织中IL-6 mRNA、JAK2 mRNA、STAT3 mRNA相对表达量高于假手术组(P<0.05);象皮Objective:To explore the effect of Xiangpi Shengji ointment on wound repair in post-operative anal fistula model rats,based on the JAK2/STAT3 signal pathway.Methods:Totally 36 SD rats were randomly divided into Xiangpi Shengji ointment group,vaseline group,sham operation group and model group,with 9 rats in each group.The model of anal fistula disease was established in rats by wire drainage,and then the anal fistula resection(except the sham operation group)was performed to establish the wound model of anal fistula surgery in rats.Rats were treated with normal saline in model group,while corresponding drugs respectively in Xiangpi Shengji ointment group and vaseline group,for 10 days.The conventional area detection method was used to detect the wound area of rats in each group on the 5th and 10th day after drug intervention.Hematoxylin-eosin(HE)staining was used to observe the pathological changes of granulation tissue on the wounds of rats in each group on the 3rd,5th,7th and 10th day after drug intervention.The expression levels of IL-6 mRNA,JAK2 mRNA and STAT3 mRNA in granulation tissues were determined by reverse transcription-polymerase chain reaction(RT-QPCR).The expression of IL6,gp130,JAK2 and STAT3 proteins in the post-anal fistula wound granulation tissues were detected by Western blotting on the 5th and 10th day after drug intervention.Results:On the 3rd day after drug intervention,the wound area of the three groups of rats was large,without redness or swelling,and there was a small amount of exudate.On the 5th,7th,and 10th day after drug intervention,the wound area decreased significantly in Xiangpi Shengji ointment group,and the model group and vaseline group showed smaller reduced wound area than Xiangpi Shengji ointment group at the same time point.On the 10th day after drug intervention,most of the wounds healed in Xiangpi Shengji ointment group,while the wounds did not fully heal in vaseline group and model group.On the 5th,7th and 10th day after drug intervention,the Xiangpi Shengji ointment gr

关 键 词：肛瘘术后 象皮生肌膏 凡士林 创面修复 JAK2/STAT3信号通路 IL-6 大鼠 

分 类 号：R285.5[医药卫生—中药学]