MTHFR、MTRR基因多态性与妊娠期高血压病的关系  

Relationship between polymorphisms of MTHFR and MIRR genes and hypertensive disorder complicating pregnancy

作　　者：刘宝阳[1] 王学超[1] LIU Baoyang;WANG Xuechao(Departrnent of Clinical Laboratory,Tianjin Baodi Hospital,Tianjin 301800,CHINA)

机构地区：[1]天津市宝坻区人民医院检验科,301800

出　　处：《江苏医药》2023年第11期1127-1130,1135,共5页Jiangsu Medical Journal

摘　　要：目的 探讨叶酸(FA)和同型半胱氨酸(Hcy)代谢过程关键酶亚甲基四氢叶酸还原酶(MTHFR)和甲硫氨酸合成酶还原酶(MTRR)基因多态性与妊娠期高血压病(HDCP)的关系。方法 采用PCR限制性内切酶多态性法检测HDCP患者104例(A组)和健康孕妇92例(C组)MTHFR基因C677T位点(MTHFR C677T)和MTRR基因A66G位点(MTRR A66G)基因多态性,采用循环酶法和微粒子化学发光法分别检测两组血清Hcy和FA水平,分析MTHFR C677T和MTRR A66G基因多态性与HDCP易感性的关系,比较A组不同基因分型血清Hcy和FA水平以及母婴结局。结果 A组MTHFR C677T基因TT基因型频率和T位点等位基因频率高于C组(P<0.05)。两组MTRR A66G基因不同基因型和等位基因频率相仿(P>0.05)。MTHFR C677T基因多态性与HDCP易感性有关,CT、TT基因型孕妇发生HDCP风险高(P<0.05)。A组MTHFR C677T基因CC、CT基因型血清Hcy水平降低,而血清FA水平升高(P<0.05)。A组MTHFR C677T基因TT基因型母婴结局差于CT、CC基因型(P<0.05)。A组MTRR A66G基因不同基因分型血清Hcy、FA水平相仿,且MTRR A66G基因不同基因分型母婴结局相仿(P>0.05)。结论 MTHFR C677T基因TT基因型是孕妇发生HDCP的高危基因型,可导致不良母婴结局。Objective To investigate the relationship between the gene polymorphisms of methylenetetrahydrofolate reductase(MTHFR),methioninesynthase reductase(MTRR),key enzymes in the metabolism of folic acid(FA)and homocysteine(Hcy),and hypertensive disorder complicating pregnancy(HDCP).Methods The polymorphism of MTHFR gene C677T site(MTHFR C677T)and MTRR gene A66G site(MTRR A66G)in 104 HDCP patients(group A)and 92 healthy pregnant women(group C)were detected by PCR restriction fragment lengthpolymorphism.Serum Hcy and FA levels of two groups were detected by circulating enzyme method and microparticle chemiluminescence method,and the relationship between the polymorphisms of MTHFR C677T and MTRR A66G genes and susceptibility to HDCP was analyzed.Serum Hcy and FA levels,maternal and infant outcomes of different genotypes in group A were compared.Results The TT genotype frequency and T allele frequency of MTHFR C677T gene in group A were higher than those in group C(P<0.05).The frequencies of different genotypes and alleles of MTRR A66G gene of two groups were similar(P>0.05).MTHFR C677T gene polymorphism was a risk factor for HDCP,in which pregnant women with CT and TT genotype had high risk of HDCP(P<0.05).Serum Hcy level of CC and CT genotypes of MTHFR C677T gene were decreased,while serum FA level was increased in group A(P<0.05).The outcome of HDCP patients with MTHFR C677T gene TT genotype was worse than those with CT and CC genotype in group A(P<0.05).Serum levels of Hcy and FA of different genotypes of MTRR A66G gene were similar,and the outcomes of the mothers and infants were similar as well in group A(P>0.05).Conclusion The TT genotype of MTHFR C677T gene is a high-risk genotype for HDCP in pregnant women,which can lead to poor maternal and infant outcomes.

关 键 词：亚甲基四氢叶酸还原酶 甲硫氨酸合成酶还原酶 妊娠期高血压病 基因多态性 

分 类 号：R714[医药卫生—妇产科学]