强还原处理配施枯草芽孢杆菌对百合土壤酚酸的影响  

Effects of Reductive Soil Disinfestation and Bacillus subtilis Inoculant on Soil Phenolic Acids of Lily

作　　者：夏青 刘子豪 张晶清 林于蓝 曾粮斌[2] 张金波[1,3,4] 蔡祖聪 赵军[1,3,4] XIA Qing;LIU Zihao;ZHANG Jingqing;LIN Yulan;ZENG Liangbin;ZHANG Jinbo;CAI Zucong;ZHAO Jun(School of Geography,Nanjing Normal University,Nanjing 210023,China;Institute of Bast Fiber Crops,Chinese Academy of Agricultural Sciences,Changsha 410205,China;Jiangsu Engineering Research Center for Soil Utilization&Sustainable Agriculture,Nanjing 210023,China;Jiangsu Center for Collaborative Innovation in Geographical Information Resource Development and Application,Nanjing 210023,China)

机构地区：[1]南京师范大学地理科学学院,南京210023 [2]中国农业科学院麻类研究所,长沙410205 [3]江苏省土壤利用与农业可持续发展工程研究中心,南京210023 [4]江苏省地理信息资源开发与利用协同创新中心,南京210023

出　　处：《土壤》2023年第5期1016-1024,共9页Soils

基　　金：国家自然科学基金项目(42090065,42077031,U21A20226);中国农业科学院科技创新工程项目(ASTIP-IBFC);江苏省研究生科研与实践创新项目(KYCX22_1569,KYCX22_1568)资助。

摘　　要：为研究不同有机物料强还原土壤处理(Reductive Soil Disinfestation,RSD)及枯草芽孢杆菌施用对土壤酚酸类物质的影响,以龙牙百合栽培土壤为研究对象,采用裂区试验设计,主区设置对照(CK)和以制糖发酵废液(MO)、植物残渣(SB)为主要有机物料的RSD处理,并于再植龙牙百合出苗后裂区添加枯草芽孢杆菌Y25(CK_Y25、MO_Y25、SB_Y25),分别在RSD处理后及龙牙百合生长期和收获期采集土壤样品,分析土壤中酚酸类物质的组成和含量变化。结果表明:与CK相比,SB处理显著(P<0.05)增加了土壤中丁香酸、对香豆酸、阿魏酸和总酚酸的含量,改变了土壤酚酸谱特征,而MO处理对土壤酚酸组成和含量的影响均不显著。龙牙百合再植能够提高RSD处理土壤中酚酸类物质含量,但不同处理随种植时间延长而呈现不同的变化规律。此外,添加枯草芽孢杆菌Y25能显著(P<0.05)降低SB处理土壤中酚酸类物质的含量(总酚酸降解率≥30%),其中对香豆酸和阿魏酸在龙牙百合生长期和收获期的降解率分别高达39.2%~47.8%和32.3%~36.7%。本研究发现,以植物残渣为有机物料的RSD处理能够提高土壤的酚酸水平,引起潜在的作物种植风险,而外源添加枯草芽孢杆菌可有效降低土壤酚酸含量,研究结果可为RSD处理的科学应用及相关配套措施的研发提供理论依据和线索。In order to clarify the effects of reductive soil disinfestation(RSD)incorporated with different organic materials and Bacillus subtilis inoculant on soil phenolic acids,a field experiment with three treatments,i.e.,CK(control without soil treatment),MO(RSD incorporated with 6 t/hm^(2)fermentation waste liquid from sugar industry),and SB(RSD incorporated with 15 t/hm^(2)plant residues),was performed on Longya Lily cultivated soils in Yuanjiang City,Hunan Province.Each soil treatment was subsequently split into two sub-plots for the inoculation of B.subtilis Y25(CK_Y25,MO_Y25,SB_Y25)after replanted Longya Lily emergence.Soil samples were collected at post-RSD treatment,growth and harvest stages of replanted Longya Lily,and high performance liquid chromatography(HPLC)was used to analyze soil phenolic acids.Results showed that SB significantly(P<0.05)altered soil phenolic acids patterns,and increased the contents of syringic acid,p-coumaric acid,ferulic acid and total phenolic acids as compared to CK,while MO had no significant effect on the composition and content of soil phenolic acids.The contents of phenolic acids in the RSD-treated soils were increased by Longya Lily replantation,but MO and SB showed different dynamic patterns with the growth of replanted Longya Lily.B.subtilis Y25 inoculant could significantly(P<0.05)reduce the contents of phenolic acids in SB-treated soil,with the degradation rate higher than 30%.In particular,the degradation rates of p-coumaric acid and ferulic acid were as high as 39.2%–47.8%and 32.3%–36.7%at the growing and harvest stages of replanted Longya Lily,respectively.In conclusion,RSD with plant residues as organic materials can increase the content of soil phenolic acids,while the introduction of exogenous microorganism with phenolic acid-degradation function can be used as an effective supporting measure to reduce soil phenolic acids after reductive soil disinfestation.

关 键 词：植物残渣 土壤酚酸 强还原土壤处理 枯草芽孢杆菌 龙牙百合 

分 类 号：S154.1[农业科学—土壤学]