不同园地土壤细菌亚群的结构与功能分异  

Structural and Functional Differentiation of Soil Bacterial Sub-communities Under Different Gardens

作　　者：喻言 刘浩 匡崇婷 巩梦梦 董雷 曹慧[1] YU Yan;LIU Hao;KUANG Chongting;GONG Mengmeng;DONG Lei;CAO Hui(College of Life Sciences/Key Laboratory of Agricultural Environmental Microbiology,Ministry of Agriculture and Rural Affairs,Nanjing Agricultural University,Nanjing 210095,China;Agricultural and Rural Bureau of Xuanzhou District,Xuancheng,Anhui 242099,China;Bengbu Cigarette Factory,China Tobacco Anhui Industrial Co.Ltd.,Bengbu,Anhui 233010,China)

机构地区：[1]南京农业大学生命科学学院/农业农村部农业环境微生物重点实验室,南京210095 [2]安徽省宣城市宣州区农业农村局,安徽宣城242099 [3]安徽中烟工业有限责任公司蚌埠卷烟厂,安徽蚌埠233010

出　　处：《土壤》2023年第5期1035-1043,共9页Soils

基　　金：国家自然科学基金项目(42077026)资助。

摘　　要：采集4种不同类型园地土壤,利用16S rRNA基因扩增子Illumina测序技术,研究不同园地土壤细菌亚群的结构与功能分异。结果表明:不同类型园地整体细菌群落多样性无显著性差异,而丰富、中等和稀有类群的α-多样性存在显著差异。对于丰富的细菌亚群,茶园和葡萄园中的多样性较高,而桃园和菜园中的多样性较低;而对于中等和稀有类群,桃园中的多样性指数较高,而菜园、茶园和葡萄园中的多样性较低。细菌群落组成分析表明,不同园地土壤中变形菌门(Proteobacteria)、酸杆菌门(Acidobacteriota)和绿弯菌门(Chloroflexi)均有较高的丰度。基于PCoA和UPGMA分析发现,细菌亚群组成在不同类型园地中均具有显著性差异,且丰富类群之间的差异大于中等和稀有类群。通过FAPROTAX预测分析发现,不同类型园地中中等和稀有类群所占据的功能种类数量比丰富类群多,且中等和稀有类群在不同类型园地之间的功能差异大于丰富类群。In this study,soils samples were collected from four types of gardens to study the structural and functional differentiation of soil bacterial sub-communities using Illumina sequencing of 16S rRNA gene amplicons.The results showed that there was no significant difference in the overall bacterial communities in the different garden soils,while there were significant differences in theα-diversity of abundant,medium and rare taxa.For the abundant bacterial sub-communities,the diversity indices were higher in tea garden and vineyard soils but lower in peach and vegetable garden soils;For the medium and rare taxa bacterial sub-communities,the diversity indices were higher in peach garden soil but lower in vegetable garden,tea garden and vineyard soils.The analysis of soil bacterial community composition showed that Proteobacteria,Acidobacteriota and Chloroflexi had high abundances in different garden soils.Based on PCoA and UPGMA analysis,it was found that bacterial sub-community compositions were significantly different in different garden soils,and the differences between abundant sub-communities were greater than those between medium and rare taxa.By FAPROTAX prediction analysis,it was found that the numbers of functional species occupied by medium and rare sub-communities were greater than those of abundant taxa in different garden soils,and the functional differences between medium and rare sub-communities were greater than those of abundant sub-communities in different garden soils.

关 键 词：园地土壤 细菌亚群 群落结构 多样性指数 功能预测 

分 类 号：S154.36[农业科学—土壤学] S572[农业科学—农业基础科学]