牛乳源致病性大肠杆菌分离鉴定、耐药性分析及快速鉴定方法的建立  

Isolation,Drug Resistance Analysis and Rapid Identification Method of Pathogenic Escherichia coli from Milk of Dairy Cow Inflicted with Mastitis

作　　者：张礼冲 ZHANG Li-chong(Gutian Featured Agricultural Seed Center,Ningde 352200)

机构地区：[1]福建古田县特色农业良种中心,宁德352200

出　　处：《中国奶牛》2023年第11期41-44,共4页China Dairy Cattle

摘　　要：为了解福建省南坪地区某规模化奶牛场乳源致病性大肠杆菌的耐药性,本研究采用K-B纸片扩散法药敏试验分析了牛乳源大肠杆菌的耐药性,并针对大肠杆菌PhoA基因设计特异性扩增引物,通过特异性和灵敏检测,建立牛乳源致病性大肠杆菌的快速鉴定方法。结果显示,75份隐性乳房炎牛乳样本中,共分离出9株致病性大肠杆菌,分离率为12%;所有菌株对β-内酰胺类、四环素类和糖肽类共6种抗菌药物具有较强的耐药性,对氨基糖苷类和喹诺酮类抗菌药物高度敏感;PhoA基因特异性引物能准确鉴定出致病性大肠杆菌,最低检定DNA浓度为0.5ng/μL。综上,本研究建议使用庆大霉素和环丙沙星对该场奶牛进行隐性乳房炎治疗,同时建立了牛乳源致病性大肠杆菌的快速鉴定方法。K-B disk diffusion test was used to analyze the drug resistance of E.coli from cow milk,and specific amplification primers were designed for E.coli PhoA gene in this study.A rapid identification method for E.coli from cow milk was established by specific and sensitive detection.The results showed that a total of 9 pathogenic Escherichia coli isolates were isolated from 75 clinical milk samples,the isolation rate was 12%.All strains had strong resistance toβ-lactam,tetracycline and glycopeptides,and were highly sensitive to aminoglycosides and quinolones.PhoA gene specific primers can accurately identify pathogenic E.coli with the lowest DNA concentration of 0.5 ng/μL.In conclusion,this study suggested the use of gentamicin and ciprofloxacin in the treatment of mastitis,and established a rapid identification method for bovine milk pathogenic E.coli.

关 键 词：乳房炎 大肠杆菌 耐药性 快速鉴定 

分 类 号：S858.23[农业科学—临床兽医学]