NEDD8在不同病理类型慢性鼻窦炎伴鼻息肉中的表达差异  

Differential expression of NEDD8 in different pathological types of chronic rhinosinusitis with nasal polyps

作　　者：孟琛 闫冰[1,2,3] 黄雨晴 王成硕 张罗[1,2,3,4] MENG Chen;YAN Bing;HUANG Yuqing;WANG Chengshuo;ZHANG Luo(Department of Otolaryngology,Head and Neck Surgery,Beijing TongRen Hospital,Capital Medical University,Beijing,100730,China;Beijing Institute of Otolaryngology,Beijing Laboratory of Allergic Diseases,Beijing Key Laboratory of Nasal Diseases,Key Laboratory of Otolaryngology Head and Neck Surgery,Ministry of Education,Capital Medical University;Research Unit of Diagnosis and Treatment of Chronic Nasal Diseases,Chinese Academy of Medical Sciences;Department of Allergy,Beijing TongRen Hospital,Capital Medical University)

机构地区：[1]首都医科大学附属北京同仁医院耳鼻咽喉头颈外科,耳鼻咽喉头颈科学教育部重点实验室(首都医科大学),北京100730 [2]北京市耳鼻咽喉科研究所,教育部工程中心,鼻病研究北京市重点实验室 [3]中国医学科学院,慢性鼻病创新单元 [4]首都医科大学附属北京同仁医院变态反应科

出　　处：《临床耳鼻咽喉头颈外科杂志》2023年第11期897-901,共5页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

基　　金：国家重点研发计划(No:2022YFC2504100);国家自然科学基金(No:82025010、81630023、82171108、81900917、81870698、82301329);教育部长江学者创新团队(No:IRT13082);中国医学科学院医学与健康科技创新工程项目资助(No:2019-I2M-5-022);北京市科学技术委员会项目(No:Z221100007422009、Z211100002921057);首都卫生发展科研专项(No:2022-1-1091);北京市医院管理局使命计划(No:SML20150203);北京市医院管理中心登峰计划(No:DFL20190202);北京市医院管理中心(No:PX2024010);东城区优秀人才计划(No:2022-dchrcpyzz-3)。

摘　　要：目的:分析不同病理类型的慢性鼻窦炎伴鼻息肉(chronic rhinosinusitis with nasal polyps,CRSwNP)患者的鼻息肉组织中神经前体细胞表达发育下调蛋白8(Neural precursor cell-expressed developmentally downregulated 8,NEDD8)表达水平差异。方法:使用在北京同仁医院耳鼻咽喉头颈外科因慢性鼻窦炎行鼻内镜手术的患者标本。利用苏木精-伊红染色检测鼻息肉组织中的嗜酸性粒细胞数量,并根据鼻息肉组织中嗜酸性粒细胞的数量对CRSwNP患者进行分组,采用免疫组织化学法检测分析鼻息肉组织中的NEDD8蛋白表达水平。结果:嗜酸性粒细胞型慢性鼻窦炎伴鼻息肉患者鼻息肉中NEDD8蛋白表达水平显著高于非嗜酸性粒细胞型慢性鼻窦炎伴鼻息肉患者(P<0.05)。NEDD8蛋白表达水平和鼻息肉组织中嗜酸性粒细胞数量呈显著正相关(r=0.79,P=0.02)。结论:不同病理类型的CRSwNP患者NEDD8蛋白表达存在差异。Objective:To analyze the differential expression of neural precursor cell-expressed developmentally downregulated 8(NEDD8)protein in nasal polyp tissues of patients with different pathological types of chronic rhinorhinosinusitis with nasal polyps(CRSwNP).Methods:All specimens were obtained from the specimen library of Beijing Tongren Hospital,and were all patients who underwent nasal endoscopic surgery for chronic rhinosinusitis in Beijing Tongren Hospital.Hematoxylin-eosin staining(HE)was used to detect the number of eosinophils in nasal polyps,and CRSwNP patients were grouped according to the number of eosinophils in nasal polyps,immunohistochemistry was used to detect and analyze the expression level of NEDD8 protein in nasal polyps.Results:The expression level of NEDD8 protein in nasal polyps of patients with eosinophilic chronic rhinorhinosinusitis with nasal polyps was significantly higher than that of patients with non-eosinophilic chronic rhinosinusitis and nasal polyps(P<0.05).In addition,there was a significant positive correlation between the expression level of NEDD8 protein and the number of eosinophils in nasal polyp tissue(r=0.79,P=0.02).Conclusion:There are differences in the expression of NEDD8 protein in patients with chronic rhinosinusitis and nasal polyps of different pathological types.

关 键 词：神经前体细胞表达发育下调蛋白8 鼻窦炎 鼻息肉 嗜酸性粒细胞 

分 类 号：R765.21[医药卫生—耳鼻咽喉科]