美登木内生真菌Neocosmospora sp.的次级代谢产物研究  

Studies on secondary metabolites of the endophytic fungus Neocosmospora sp.from Maytenus hookeri Loes.

作　　者：王晓晴 刘博 翁小刚[1] 王琪 陈育伍 牟越 孙奕[1] WANG Xiao-qing;LIU Bo;WENG Xiao-gang;WANG Qi;CHEN Yu-wu;MU Yue;SUN Yi(Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China)

机构地区：[1]中国中医科学院中药研究所,北京100700

出　　处：《中国药物化学杂志》2023年第11期849-856,共8页Chinese Journal of Medicinal Chemistry

基　　金：中国中医科学院科技创新工程项目(CI2021A04514)。

摘　　要：目的对美登木内生真菌Neocosmospora sp.中抗肿瘤活性次级代谢产物进行研究。方法结合LC-MS分析与MTT活性检测方法,采用反相ODS、反复硅胶柱色谱、高效液相色谱等方法对活性次级代谢产物进行分离纯化,并根据理化性质与波谱数据鉴定化合物结构。结果与结论从美登木内生真菌总次级代谢产物中分离得到12个化合物,分别鉴定为cyclotryprostatin B(1)、fumitremorgin C(2)、bisdethiobis(methylthio)gliotoxin(3)、spirotryprostatin A(4)、(6S,14S)-14,15-dihydro-6-methyl-6,14-(iminomethano)-5H-indolo-[2′,3′:4,5]azepino[2,1-b]quinazoline-12,15(6H)-dione(5)、fumiquinazoline C(6)、pseurotin A(7)、5α,8α-epidi-oxy-(22E,24R)-ergosta-6,22-dien-3β-ol(8)、5α,8β-epidioxy-24(S)-methylcholesta-6,9(11),22-triene-3β-ol(9)、(22E,24R)-3β,5α,9α-trihydroxyergosta-7,22-diene-6-one(10)、stigmasta-7,22-diene-3β,5α,6α-triol(11)、4-hydroxyphenethyl-2′(S)-hydroxypropanoate(12)。所有化合物均为首次从内生真菌Neocosmospora属中分离得到,化合物4对肺癌A549细胞具有较强的增殖抑制活性(IC^(50)=5.1μmol·L^(-1))。An endophytic fungus Neocosmospora sp.was screened based on LC-MS analysis and MTT activity detection.Twelve secondary metabolites were isolated and purified by using ODS flash column,thin layer chromatography,silica gel column chromatography and high performance liquid chromatography.The compounds were identified as cyclotryprostatin B(1),fumitremorgin C(2),bisdethiobis(methylthio)gliotoxin(3),spirotryprostatin A(4),(6S,14S)-14,15-dihydro-6-methyl-6,14-(iminomethano)-5H-indolo-[2',3':4,5]azepino[2,1-b]quinazoline 12,15(6H)-dione(5),fumiquinazoline C(6),pseurotin A(7),5x.,8xx-epidioxy-(22E,24R).-ergosta-6,22-dien 3B-ol(8),5a,8βepidioxy-24(S)-methylcholesta-6,9(11),22-triene 3β-ol(9),(22E,24R)-3β,5c,9a-trihydroxyergosta-7,22-diene-6--one(10),stigmasta-7,22-diene-3β,5a,6a-triol(11),4-hydroxyphenethy1-2'(S)-hydroxypropanoate(12)by extensive spectroscopic analysis.All the compounds were isolated from the endophytic fungus Neocosmospora sp.for the first time,and compound 4 showed strong proliferative inhibitory activity against the lung cancer cell line A549 with the IC^(50)value of 5.1μmol·L^(-1).

关 键 词：美登木 内生真菌 Neocosmospora sp. 次级代谢产物 抗肿瘤活性 

分 类 号：R284[医药卫生—中药学]