血清miR-132、miR-214、HMGB1表达与急性心肌梗死患者经皮冠脉介入术后MACE发生的关系研究  

Relationship between Serum miR-132,miR-214,HMGB1 and MACE in Patients with Acute Myocardial Infarction after Percutenous Coronary Intervention

作　　者：刘曙杰 孙立娜 韩丹丹 陈儒 韩晓庆 王均志 王杰[2] LIU Shujie;SUN Lina;HAN Dandan;CHEN Ru;HAN Xiaoqing;WANG Junzhi;WANG Jie(Department of Cardiovascular Medicine,People's Hospital of Chengyang District,Qingdao 266109,Shandong,China;Department of Cardiology,The First Affiliated Hospital of Hunan University of Chinese Medicine,Changsha 410021,Hunan,China)

机构地区：[1]青岛市城阳区人民医院心血管内科,青岛市266109 [2]湖南中医药大学第一附属医院心内科,长沙市410021

出　　处：《中国分子心脏病学杂志》2023年第5期5657-5662,共6页Molecular Cardiology of China

基　　金：湖南省科技计划项目(2019011)。

摘　　要：目的分析血清miR-132、miR-214、高迁移率族蛋白B1(HMGB1)表达与急性心肌梗死(AMI)患者经皮冠脉介入术(PCI)后心血管不良事件(MACE)发生的关系。方法选取2021年1月至2022年1月收治的106例AMI患者作为AMI组,行PCI,均随访6个月,统计患者MACE发生情况,将发生MACE的患者纳入MACE组,反之纳入非MACE组。采用实时荧光定量检测血清中miR-132、miR-214水平,酶联免疫吸附法检测血清中HMGB1水平,化学发光法检测血清心肌肌钙蛋白I(cTnI),免疫透射比浊法测定血清超敏C反应蛋白(hs-CRP)水平,采用超声心动图检测心功能。绘制受试者操作特征(ROC)曲线,计算曲线下面积(AUC),评估术前血清miR-132、miR-214和HMGB1对AMI患者MACE的预测价值。结果AMI患者PCI术后血清中miR-132、miR-214、左心室射血分数(LVEF)水平明显高于术前(P<0.05)。106例AMI患者经6个月随访后有32例发生MACE。MACE组AMI患者cTnI、hs-CRP、左室舒张末期内径(LVEDD)水平高于非MACE组,LVEF低于非MACE组(P<0.05)。MACE组AMI患者PCI术后血清中miR-132、miR-214 mRNA水平明显低于非MACE组(P<0.05),HMGB1 mRNA水平明显高于非MACE组(P<0.05)。ROC曲线分析显示,miR-132、miR-214、HMGB1及联合检测预测AMI患者预后的AUC分别为0.713、0.724、0.713、0.787(P<0.05)。结论AMI患者PCI治疗后血清中miR-132、miR-214水平下降,HMGB1水平上升,上述指标联合检测能预测AMI患者PCI治疗后的MACE发生情况。Objective To analyze the relationship between serum miR-132,miR-214,high mobility group B1(HMGB1)and major adverse cardiovascular events(MACE)in patients with acute myocardial infarction(AMI)after percutaneous coronary intervention(PCI).Methods A total of 106 patients with AMI admitted to the hospital were enrolled as AMI group between January 2021 and January 2022.All underwent PCI and then were followed up for 6 months to statistically analyze the occurrence of MACE.Patients with MACE were included in MACE group,while those without MACE were included in non-MACE group.The levels of serum miR-132 and miR-214 were detected by real-time fluorescence quantitation method.The level of serum HMGB1 was detected by enzyme-linked immunosorbent assay.The level of serum cardiac troponin I(cTnI)was detected by chemiluminescence method.The level of serum hypersensitive C-reactive protein(hs-CRP)was detected by immunity transmission turbidity.The cardiac function was tested by echocardiography.The predictive value of preoperative serum miR-132,miR-214 and HMGB1 for MACE was evaluated by area under curves(AUC)of receiver operating characteristic(ROC).Results After PCI,levels of serum miR-132,miR-214 and left ventricular ejection fraction(LVEF)were significantly increased(P<0.05).In the 106 AMI patients after 6 months of followup,there were 32 cases with MACE.The levels of cTnI,hsCRP and left ventricular end diastolic diameter(LVEDD)in MACE group were higher than those in non-MACE group,while LVEF was lower than that in non-MACE group(P<0.05).After PCI,levels of serum miR-132 and miR-214 in MACE group were significantly lower than those in non-MACE group(P<0.05),while mRNA level of HMGB1 was significantly higher than that in non-MACE group(P<0.05).ROC curves analysis showed that AUC values of miR-132,miR-214,HMGB1 and combined detection for predicting prognosis of AMI patients were 0.713,0.724,0.713 and 0.787,respectively(P<0.05).Conclusion The levels of serum miR-132 and miR-214 decrease,while HMGB1 increases in AMI patients a

关 键 词：急性心肌梗死 经皮冠脉介入术 miR-132 miR-214 高迁移率族蛋白B1 心血管不良事件 

分 类 号：R542.22[医药卫生—心血管疾病]