Isolation, characterization and modulatory potentials of β-stigmasterol, ergosterol and xylopic acid from Anchomanes difformis on mitochondrial permeability transition pore in vitro  

作　　者：Kehinde Oluseun Sodeinde Akinwunmi Oluwaseun Adeoye Adedeji Adesipo Adebayo AAdeniyi John Adeolu Falode Tajudeen Olabisi Obafemi Samuel Olalekan Olusanya Linette Twigge Jeanet Conradie Timothy O.Mosaku 

机构地区：[1]Department of Chemistry,Federal University Oye-Ekiti,Ekiti State 371104,Nigeria [2]Biomembrane,Toxicology and Drug Discovery Unit,Department of Biochemistry,Federal University Oye-Ekiti,Ekiti State 371104,Nigeria [3]Department of Biochemistry,Afe Babalola University,Ado-Ekiti,Ekiti State 360001,Nigeria [4]Department of Chemistry,University of Free State,Bloemfontein 9301,South Africa [5]Department of Civil Engineering,Covenant University,Ogun State 112104,Nigeria

出　　处：《Chinese Herbal Medicines》2023年第4期533-541,共9页中草药（英文版）

摘　　要：Objective: Secondary metabolites and polyphenolic compounds from medicinal plants have been demonstrated to have multiple biological functions with promising research and development prospects. This study examined the effect of β-stigmasterol(with ergosterol) and xylopic acid isolated from Anchomanes difformis on liver mitochondrial permeability transition pore(mPTP).Methods: The compounds were isolated by vacuum liquid chromatography. Mitochondrial swelling was assessed as changes in absorbance under succinate-energized conditions.Results:1H and13C NMR spectroscopic elucidation of the isolates affirmed the presence of β-stigmasterol with ergosterol(1:0.3) and xylopic acid. The isolates reversed the increase in lipid peroxidation and inhibited the opening of mitochondrial permeability transition pores caused by calcium and glucose.Pharmacological inhibition of m PTP offers a promising therapeutic target for the treatment of mitochondrial-associated disorders.Conclusion: Reduction in the activity of calcium ATPase and the expression of Caspase-3 and-9 were observed, suggesting that they could play a role in protecting physicochemical properties of membrane bilayers from free radical-induced severe cellular damage and be useful in the management of diseases where much apoptosis occurs.

关 键 词：Anchomanes difformis(Blume)Engl ERGOSTEROL mitochondrial membrane MODULATION β-stigmasterol transition pore xylopic acid 

分 类 号：R284[医药卫生—中药学]