Transcriptome analysis of Saposhnikovia divaricata and mining of bolting and fowering genes  

作　　者：Min Zhang Wenle Wang Qian Liu Erhuan Zang Lijun Wu Guofa Hu Minhui Li 

机构地区：[1]Inner Mongolia Hospital of Traditional Chinese Medicine,Hohhot 010020,China [2]Baotou Medical College,Baotou 014040,China [3]Inner Mongolia Traditional Chinese&Mongolian Medical Research Institute,Hohhot 010010,China [4]Hulunbuir Hengyi Chinese Medicinal Plants Co.,Ltd.,Hulunbuir 021099,China

出　　处：《Chinese Herbal Medicines》2023年第4期574-587,共14页中草药（英文版）

基　　金：supported by China Agriculture Research System of MOF and MARA (No. CARS-21)。

摘　　要：Objective: Early bolting of Saposhnikovia divaricata has seriously hindered its medicinal value and sustainable development of resources. The molecular mechanism of bolting and fowering of S. divaricata is still unclear and worth of research. In our study, we explored the transcriptome of the genes related to the bolting and fowering of S. divaricata.Methods: The transcriptome library was constructed, sequenced, assembled and annotated from the bolting and unbolting leaves of S. divaricata by high-throughput sequencing at the bud and fowering stage.Focus on the pathways related to bolting and fowering in plants, and exploring genes. The expression of seven candidate genes was verified by real-time fuorescence quantitative PCR(qRT-PCR).Results: Transcriptome results showed that 249 889 422 high-quality clean reads were obtained. A total of 67 866 unigenes were assembled with an average length of 948.1 bp. Trinity de Novo assembly produced 67 866 unigenes with an average length of 948.1 bp. Among 993 differentially expressed genes,484 genes were significantly up-regulated and 509 genes were down-regulated in the SdM group. A total of 79 GO terms were significantly enriched for differentially expressed genes. KEGG results showed that 11 154 unigenes were enriched in 89 pathways. And 21 candidate genes related to bolting and fowering of S. divaricata were excavated. The qRT-PCR results showed that expression trends of HDA9, PHYB, AP2,TIR1, Hsp90, CaM, and IAA7 were consistent with transcriptomic sequencing results. In addition, RNA-seq had identified 10 740 transcription factors and classified them into 58 families by their conserved domains. Further studies showed that the transcription factors regulating the fowering of S. divaricata were mainly distributed in the NAC, MYB_related, HB-other, ARF, and AP2 families.Conclusion: Based on the results of this study, it was found that the plant hormone signal transduction pathway was one of the decisive factors to control bolting and fowering. Among them, auxin related ge

关 键 词：bolting and bloom comparative analysis flowering genes Saposhnikovia divaricata(Turcz.)Schischk TRANSCRIPTOME 

分 类 号：S567.239[农业科学—中草药栽培]