Determination of Oleuropein in Cosmetics by Ultra Performance Liquid Chromatography-Triple Quadrupole Tandem Mass Spectrometry  

作　　者：Pan Lijing Chen Weiwei Yuan Minjia 

机构地区：[1]Shanghai Qiran Biotechnology Co.,Ltd.,China [2]Shanghai Jinjia Technology Co.,Ltd.,China

出　　处：《China Detergent & Cosmetics》2023年第4期30-36,共7页日用化学品科学（英文版）

摘　　要：An ultrahigh performance liquid chromatography-triple quadrupole tandem mass spectrometry(UPLC-MS/MS)was established to quickly and accurately determine the content of oleuropein in cosmetics.The samples were extracted with methanol-aqueous solution,and the mobile phase with methanol-formic acid solution(0.1 mol/L)=40∶60 was separated by Agilent ZORBAX Eclipse Plus C18(2.1 mm×50 mm×1.8μm-Micron)column temperature 30℃,flow rate 0.3 mL/min.The MS end was detected by electrospray negative mode ionization(ESI-)and multiple reaction monitoring(MRM)mode.The results show a good linear relationship in the range of 0.002~5 mg/L,with a correlation coefficient R2 of 0.999,5.Method recovery range from 84.2%~107.6%and the relative standard deviation RSD is 5.8%.The detection time is 5 min,the detection limit is 0.000,6 mg/L,and the limit of quantification is 0.002 mg/L.This method has the advantages of convenient operation,low quantification limit,high precision and good repeatability,and is suitable for measuring the content of oleuropein in many kinds of cosmetics.

关 键 词：ultra performance liquid chromatography-triple quadrupole tandem mass spectrometry(UPLC-MS/MS) OLEUROPEIN COSMETICS 

分 类 号：O657.63[理学—分析化学] TQ658[理学—化学]