禁欲时间对精子DNA损伤、形态及精液常规分析参数的影响  

Effects of abstinence time on the parameters of sperm DNA damage,morphology and routine semen analysis

作　　者：葛燕梅 陆金春 徐院花 唐山山 梁元姣 Ge Yanmei;Lu Jinchun;Xu Yuanhua;Tang Shanshan;Liang Yuanjiao(Center for Reproductive Medicine,Zhongda Hospital,Southeast University,Nanjing 210037,Jiangsu,China)

机构地区：[1]东南大学附属中大医院生殖医学中心,江苏南京210037

出　　处：《中国男科学杂志》2023年第5期57-62,共6页Chinese Journal of Andrology

基　　金：南京市卫健委卫生科技发展专项资金项目(YKK21271)。

摘　　要：目的探讨不同禁欲时间对精子DNA损伤、形态及精液常规分析参数的影响。方法收集从2018年9月至2021年2月到东南大学附属中大医院生殖医学中心就诊的男性患者精液样本1128例。采用CFT-9201型计算机辅助精子分析系统进行精液常规分析,包括精子浓度、精子活动率、前向运动精子百分率(PR)等主要参数;采用Diff-Quik染色法进行精子形态分析;采用精子染色质结构分析法(SCSA)检测精子DNA碎片指数(DFI)。结果1128例患者的禁欲时间为0~30天,中位数为3.0[3.0,5.0];仅精子活动率呈正态分布(P=0.117),其余参数均呈非正态分布(P<0.01)。患者禁欲时间与精液量(r=0.21,P<0.01)、精子浓度(r=0.098,P<0.01)和精子DFI(r=0.068,P<0.05)均呈显著正相关,而与PR、精子活动率和正常形态精子百分率均呈负相关,但均无显著相关性(P>0.05)。精子DFI与精子浓度没有显著相关性(P>0.05),但与PR、精子活动率和正常形态精子百分率均呈显著负相关(P<0.01)。结论随着禁欲时间的延长,精液量和精子浓度均增加,DFI也随之上升,故适当缩短禁欲时间可以减少精子DNA碎片的产生。临床医生在向患者解释精液分析结果时应考虑禁欲时间的影响。Objective To investigate the effects of different abstinence time on the parameters of sperm DNA damage,morphology and routine semen analysis.Methods A total of 1128 semen samples were collected from male patients in the Center for Reproductive Medicine,Zhongda Hospital,Southeast University during September 2018 and February 2021.Routine semen analysis parameters,including sperm concentration,sperm motility,percentage of progressively motile sperm(PR),etc.,were detected by the CFT-9201 computer-aided sperm analysis(CASA)system.Sperm morphology was observed after Diff-Quik staining,and sperm DNA fragmentation index(DFI)was detected by sperm chromatin structure analysis(SCSA).Results Abstinence time of 1128 patients ranged from 0 to 30 days,with a median of 3.0[3.0,5.0].In addition to sperm motility(P=0.117)showed normal distribution,other parameters all showed non normal distribution(P<0.01).Abstinence time was positively correlated with semen volume(r=0.21,P<0.01),sperm concentration(r=0.098,P<0.01)and sperm DFI(r=0.068,P<0.05).Although abstinence time was negatively correlated with PR,sperm motility and percentage of normal morphology sperm,there was no significant correlation(P>0.05).Sperm DFI was negatively correlated with PR,sperm motility and percentage of normal sperm morphology(P<0.01),but not with sperm concentration(P>0.05).Conclusion With the extension of abstinence time,semen volume and sperm concentration increase,and sperm DFI also increases.Therefore,appropriately shortening abstinence time can reduce the production of sperm DNA fragments.Clinicians should consider the influence of abstinence time when explaining semen analysis results to patients.

关 键 词：禁欲时间 精液分析 精子 DNA碎片 

分 类 号：R321.1[医药卫生—人体解剖和组织胚胎学]