Efficient CRISPR/Cas9-mediated genome editing in sheepgrass(Leymus chinensis)  

作　　者：Zhelong Lin Lei Chen Shanjie Tang Mengjie Zhao Tong Li Jia You Changqing You Boshu Li Qinghua Zhao Dongmei Zhang Jianli Wang Zhongbao Shen Xianwei Song Shuaibin Zhang Xiaofeng Cao 

机构地区：[1]State Key Laboratory of Plant Genomics and National Center for Plant Gene Research,Institute of Genetics and Developmental Biology,The Chinese Academy of Sciences,Beijing 100101,China [2]Institute of Forage and Grassland Sciences,Heilongjiang Academy of Agricultural Sciences,Harbin 150086,China [3]State Key Laboratory of Plant Cell and Chromosome Engineering,Center for Genome Editing,Institute of Genetics and Developmental Biology,The Chinese Academy of Sciences,Beijing 100101,China

出　　处：《Journal of Integrative Plant Biology》2023年第11期2416-2420,共5页植物学报（英文版）

基　　金：supported by Key Projects in Science and Technology of Inner Mongolia (2021ZD0031);the Strategic Priority Research Program of the Chinese Academy of Sciences (XDA26030202);the Youth Innovation Promotion Association,CAS (2022096)。

摘　　要：The lack of genome editing platforms has hampered efforts to study and improve forage crops that can be grown on lands not suited to other crops.Here,we established efficient Agrobacterium-mediated clustered regularly interspaced palindromic repeats(CRISPR)/CRISPR-associated nuclease 9(Cas9)genome editing in a perennial,stress-tolerant forage grass,sheepgrass(Leymus chinensis).By screening for active single-guide RNAs(sg RNAs),accessions that regenerate well,suitable Agrobacterium strains,and optimal culture media,and co-expressing the morphogenic factor Ta WOX5,we achieved 11%transformation and 5.83%editing efficiency in sheepgrass.Knocking out Teosinte Branched1(TB1)significantly increased tiller number and biomass.This study opens avenues for studying gene function and breeding in sheepgrass.

关 键 词：AGROBACTERIUM genetic transformation genome editing Lc TB1 sheepgrass 

分 类 号：S543.9[农业科学—作物学] Q943.2[生物学—植物学]