The DEAD-box RNA helicase ZmRH48 is required for the splicing of multiple mitochondrial introns,mitochondrial complex biosynthesis,and seed development in maize  

作　　者：Yan-Zhuo Yang Shuo Ding Xin-Yuan Liu Chunhui Xu Feng Sun Bao-Cai Tan 

机构地区：[1]Key Laboratory of Plant Development and Environmental Adaptation Biology,Ministry of Education,School of Life Sciences,Shandong University,Qingdao 266237,China

出　　处：《Journal of Integrative Plant Biology》2023年第11期2456-2468,共13页植物学报（英文版）

基　　金：supported by the National Natural Science Foundation of China (Project Nos.32072126 and 32230075);the Shandong Provincial Natural Science Foundation (Project No.ZR2019MC005)。

摘　　要：RNA helicases participate in nearly all aspects of RNA metabolism by rearranging RNAs or RNA–protein complexes in an adenosine triphosphatedependent manner.Due to the large RNA helicase families in plants,the precise roles of many RNA helicases in plant physiology and development remain to be clarified.Here,we show that mutations in maize(Zea mays)DEAD-box RNA helicase48(Zm RH48)impair the splicing of mitochondrial introns,mitochondrial complex biosynthesis,and seed development.Loss of Zm RH48 function severely arrested embryogenesis and endosperm development,leading to defective kernel formation.Zm RH48 is targeted to mitochondria,where its deficiency dramatically reduced the splicing efficiency of five cis-introns(nad5 intron 1;nad7 introns 1,2,and 3;and ccm Fc intron 1)and one trans-intron(nad2 intron 2),leading to lower levels of mitochondrial complexes I andⅢ.Zm RH48 interacts with two unique pentatricopeptide repeat(PPR)proteins,PPR-SMR1 and SPR2,which are required for the splicing of over half of all mitochondrial introns.PPR-SMR1 interacts with SPR2,and both proteins interact with P-type PPR proteins and Zm-m CSF1 to facilitate intron splicing.These results suggest that Zm RH48 is likely a component of a splicing complex and is critical for mitochondrial complex biosynthesis and seed development.

关 键 词：intron splicing MAIZE mitochondria RNA helicase seed development ZmRH48 

分 类 号：S513[农业科学—作物学]