A loss-of-function mutant allele of a glycosyl hydrolase gene has been co-opted for seed weight control during soybean domestication  

作　　者：Siming Wei Bin Yong Hongwei Jiang Zhenghong An Yan Wang Bingbing Li Ce Yang Weiwei Zhu Qingshan Chen Chaoying He 

机构地区：[1]State Key Laboratory of Plant Diversity and Specialty Crops/State Key Laboratory of Systematic and Evolutionary Botany,Institute of Botany,the Chinese Academy of Sciences,Beijing 100093,China [2]China National Botanical Garden,Beijing 100093,China [3]College of Life Sciences,University of Chinese Academy of Sciences,Beijing 100049,China [4]College of Agriculture,Northeast Agricultural University,Harbin 150030,China [5]Jilin Academy of Agricultural Sciences,Changchun 130022,China [6]The Innovative Academy of Seed Design,the Chinese Academy of Sciences,Beijing 100101,China

出　　处：《Journal of Integrative Plant Biology》2023年第11期2469-2489,共21页植物学报（英文版）

基　　金：supported by the grants from the Chinese Academy of Sciences (ZDRW-ZS-2019-2-0101 and XDA08010105);the National Natural Science Foundation of China (31525003,31930007)to C.Y.H。

摘　　要：The resultant DNA from loss-of-function mutation can be recruited in biological evolution and development.Here,we present such a rare and potential case of“to gain by loss”as a neomorphic mutation during soybean domestication for increasing seed weight.Using a population derived from a chromosome segment substitution line of Glycine max(SN14)and Glycine soja(ZYD06),a quantitative trait locus(QTL)of 100-seed weight(q HSW)was mapped on chromosome 11,corresponding to a truncatedβ-1,3-glucosidase(βGlu)gene.The novel gene hsw results from a 14-bp deletion,causing a frameshift mutation and a premature stop codon in theβGlu.In contrast to HSW,the hsw completely lostβGlu activity and function but acquired a novel function to promote cell expansion,thus increasing seed weight.Overexpressing hsw instead of HSW produced large soybean seeds,and surprisingly,truncating hsw via gene editing further increased the seed size.We further found that the core 21-aa peptide of hsw and its variants acted as a promoter of seed size.Transcriptomic variation in these transgenic soybean lines substantiated the integration hsw into cell and seed size control.Moreover,the hsw allele underwent selection and expansion during soybean domestication and improvement.Our work cloned a likely domesticated QTL controlling soybean seed weight,revealed a novel genetic variation and mechanism in soybean domestication,and provided new insight into crop domestication and breeding,and plant evolution.

关 键 词：DOMESTICATION frameshift mutation QTL seed weight SOYBEAN to gain by loss 

分 类 号：S565.1[农业科学—作物学]