基于GEO数据库的热射病神经损伤相关基因的生物信息学分析  

Bioinformatic analysis of genes related to nerve injury in heat stroke based on GEO database

作　　者：王蕾 姜岱山 朱保锋[1] 贾寒雨 沈君华[1] 张毅[4] Wang Lei;Jiang Daishan;Zhu Baofeng;Jia Hanyu;Shen Junhua;Zhang Yi(Emergency Center of the Second Affiliated Hospital of Nantong University,Nantong 226001,China;Emergency Center of the Affiliated Hospital of Nantong University,Nantong 226001,China;Department of Science and Technology,the Second Afiliated Hospital of Nantong University,Nantong 226001,China;Department of Neurosurgery,the Second Affliated Hospital of Nantong University,Nantong 226001,China)

机构地区：[1]南通大学第二附属医院急诊中心,江苏南通226001 [2]南通大学附属医院急诊中心,江苏南通226001 [3]南通大学第二附属医院科技处,江苏南通226001 [4]南通大学第二附属医院神经外科,江苏南通226001

出　　处：《中华神经创伤外科电子杂志》2023年第2期76-84,共9页Chinese Journal Of Neurotraumatic Surgery：Electronic Edition

基　　金：江苏省卫生和健康委员会医学研究项目(Z2022067);江苏省医院急诊管理专项课题(JSYGY-3-2021-JZ28);南通市科技局研究项目(JC2021179);南通市卫生和健康委员会科研课题(MB2021018、MB2021026)。

摘　　要：目的通过生物信息学检索热射病神经损伤的相关基因,探究热射病神经损伤的可能分子机制并进行初步验证。方法(1)以2022年4月作为时间截点,基于基因表达数据库(GEO)筛选热射病神经损伤相关基因表达谱数据,通过检索数据集GSE64778获得差异表达基因(DEGs),结合GeneCards和CTD数据库检索热射病相关靶基因,取交集筛选获得候选靶基因。采用R语言的"ClusterProfiler"包对热射病相关候选靶基因进行GO和KEGG富集分析;进一步使用STRING数据库构建靶基因的交互作用网络图,并对关键基因进行相关性分析获得核心基因。(2)收集江苏省南通市第一人民医院自2022年6~8月收治的热射病患者的外周血,通过PCR技术进行核心基因的表达验证。结果(1)GSE64778数据集的SRA数据筛选,共获得1178个DEGs,其中322个上调,856个下调。GeneCards数据库筛选出2914个基因,CTD数据库筛选出2377个基因。将GSE64778数据集差异表达排名前300的基因,与GeneCards和CTD数据库检索结果取交集,最终筛选出25个候选DEGs。(2)GO功能注释结果显示靶基因主要参与细胞凋亡、应激反应以及细胞过程的负调控,在蛋白质二聚化和蛋白结合等过程中发挥功能。KEGG通路富集分析显示候选靶基因主要富集在PI3K-Akt信号通路。PPI网络分析显示Hsp90AA1是Degree值最高的枢纽基因,且在PI3K-Akt信号通路中富集。(3)经qRT-PCR验证,Hsp90AA1在热射病早期神经损伤患者中的表达均与生物信息学分析结果一致。结论Hsp90AA1可能为热射病神经损伤主要的影响基因,与细胞凋亡、应激反应以及细胞过程的负调控相关。Objective To search for genes related to nerve injury in heat stroke(HS)by bioinformatics,the possible molecular mechanism of nerve injury in HS was explored and preliminarily verified.Methods(1)Taking April 2022 as the time cut-off,the expression profile datas of nerve inject-related genes in thermal radiation disease were screened based on GEO and other databases,and differentially expressed genes(DEGs)were obtained by retrieving datas set GSE64778,combined with GeneCards and CTD databases to retrieve HS related target genes,and the candidate target genes were obtained by intersection screening.The"ClusterProfiler"package in R language was used to perform GO and KEGG enrichment analysis for HS related target genes;STRING database was further used to construct the interaction network map of target genes and correlation analysis of key genes to obtain core genes.(2)Peripheral blood of patients with HS treated in Nantong First People's Hospital of Jiangsu Province from June 2022 to August 2022 were collected,and the expression of core genes were verified by PCR technology.Results(1)SRA datas screening of the GSE64778 dataset yielded 1178 DEGs,of which 322 were up-regulated and 856 were down-regulated.2914 genes were screened in the GeneCards database and 2377 genes were screened in the CTD database.The top 300 differentially expressed genes in the GSE64778 dataset were intersected with the search results of GeneCards and CTD databases,and 25 candidate DEGs were finally screened.(2)GO functional annotation results indicated that the target genes were mainly involved in apoptosis,stress response and negative regulation of cellular processes,and functioned in processes such as protein dimerization and protein binding.KEGG pathway enrichment analysis suggested that the candidate target genes were mainly enriched in PI3K-Akt signaling pathway.PPI network analysis suggested that Hsp90AA1 was the hub gene with the highest Degree value and enriched in PI3K-Akt signaling pathway.(3)qRT-PCR confirmed that the expression of

关 键 词：热射病 神经损伤 生物信息学 Hsp90AA1 PI3K-AKT信号通路 

分 类 号：R594.12[医药卫生—内科学]