RNA Imaging in Live Cells  

作　　者：Jingfang Zhao Limin Xiang 

机构地区：[1]College of Chemistry and Molecular Sciences&TaiKang Center for Life and Medical Sciences,Wuhan University,Wuhan,Hubei,430072 China

出　　处：《Chinese Journal of Chemistry》2023年第19期2531-2535,共5页中国化学（英文版）

基　　金：supports from National Key R&D Pro-gram of China(Grant 2022YFA1305400);National Natural Sci-ence Foundation of China(Grant 22104113,Grant 22274122).

摘　　要：Traditional molecular biology tools have elucidated the identities and functions of RNA molecules,which are essential to the understanding of gene transcription and protein translation.Deepening this research field would further require the direct visualization of RNA dynamics such as the DNA-RNA interactions and RNA-protein interactions.Towards this goal,the rise of RNA imaging tools over the past 15 years has reformed how we looked at these processes.In this Emerging Topic,we first highlighted recent advances on three main RNA imaging tools based on the species of interacting molecules:RNA-RNA pairing,RNA-protein binding,and small molecule-RNA complex.We introduced the advantages of these tools from a technical viewpoint,including binding affinity,fluorescent turn-on ratio,stability,and impacts on targeted RNA.Next,we discussed new rising opportunities and future directions,echoing the state-of-the-art imaging tools in the fields of fluorescent proteins and small fluorescent molecules.Together,we believe this emerging field will bring new insights on how we study RNA biology in living systems.

关 键 词：Fluorescent imaging RNA imaging Fluorogenic RNA RNA Fluorescent probes RNA recognition 

分 类 号：O657.3[理学—分析化学]