IL-17、TGF-β在老年细菌性肺炎患者血清和肺泡灌洗液中的表达水平及临床价值研究  

The expression and clinical significance of IL-17,TGF-βin serum and bronchoalveolar lavage fluid of elderly patients with bacterial infection pneumonia

作　　者：林洁 林树云 林喜雄 黄志伟 余进胜 Lin Jie;Lin Shuyun;Lin Xixiong;Huang Zhiwei;Yu Jinsheng(Huidong County People's Hospital,Huizhou,Guangdong 516300,China)

机构地区：[1]广东省惠东县人民医院,广东惠州516300

出　　处：《首都食品与医药》2023年第23期83-85,共3页Capital Food Medicine

基　　金：惠州市科技计划项目(2022CZ010353);广东省基础与应用基础研究基金项目(2022A1515012640)。

摘　　要：目的探讨老年细菌性肺炎(BP)患者血清和肺泡灌洗液(BALF)中的IL-17、TGF-β表达水平及临床意义。方法将2022年8月-2023年2月期间惠东县人民医院收治的60例老年BP患者纳入实验组,并根据病情严重程度分为重症组和非重症组各30例,另选同期在该院体检的30例健康老年受试者纳入对照组。采用双抗体夹心法酶联免疫吸附试验(ELISA)检测血清及BALF中IL-17和TGF-β的表达;Pearson相关性分析老年BP患者血清及BALF中IL-17和TGF-β的相关性。ROC曲线分析血清IL-17、TGF-β及二者联合检测对老年BP发生的诊断价值。结果实验组血清IL-17、TGF-β分别为(181.36±19.27)pg/mL、(121.25±16.74)ng/mL,较对照组的(45.26±16.11)pg/mL、(34.19±8.92)ng/mL显著增加(P<0.05);重症组血清和BALF中的IL-17、TGF-β分别为(233.45±15.22)pg/mL、(129.74±14.05)ng/mL、(262.42±19.79)pg/mL、(154.36±16.17)ng/mL,较非重症组的(161.38±17.10)pg/mL、(108.36±13.14)ng/mL、(184.02±17.34)pg/mL、(124.26±15.40)ng/mL显著增加(P<0.05);Pearson相关性分析显示,血清和BALF中的IL-17与TGF-β均呈正相关(r=0.497、0.511,P<0.05);ROC曲线分析显示,血清IL-17、TGF-β及二者联合检测对老年BP诊断价值的曲线下面积(AUC)分别为0.819、0.825和0.920。结论IL-17、TGF-β在老年BP患者中表达显著上调,且IL-17、TGF-β呈正相关,与疾病的严重程度相关联,二者联合检测对老年BP的诊断价值较高。Objective To investigate the expression and clinical significance of IL-17 and TGF-βin serum and alveolar lavage fluid(BALF)of elderly patients with bacterial pneumonia(BP).Methods 60 elderly patients with BP admitted to Huidong County People's Hospital from August 2022 to February 2023 were included in the experimental group,and were divided into severe group and non severe group according to the severity of the disease,30 healthy elderly patients examined in our hospital at the same time were included in the control group.IL-17 and TGF in serum and BALF-βwere detected using ELISA.The correlation of IL-17 with TGF-βin serum and BALF of elderly patients wiht BP was analyzed by Pearson correlation analysis.Diagnostic value of serum IL-17 and TGF-βand their combination detection for the occurrence of BP in the elderly was estimated by ROC analysis.Result Serum IL-17 and TGF-βin the experimental group was(181.36±19.27)pg/mL and(121.25±16.74)ng/mL,respectively,significantly increased than those of the control group,which was(45.26±16.11)pg/mL and(34.19±8.92)ng/mL(P<0.05).Serum and BALF IL-17 and TGF-βof the severe group was(233.45±15.22)pg/mL,(129.74±14.05)ng/mL,(262.42±19.79)pg/mL,and(154.36±16.17)ng/mL,respectively,significantly increased than those of the non-severe group,which was(161.38±17.10)pg/mL,(108.36±13.14)ng/mL,(184.02±17.34)pg/mL,and(124.26±15.40)ng/mL in(P<0.05).Pearson correlation analysis showed that serum and BALF IL-17 was positive correlation with TGF-β(r=0.497,0.511,P<0.05).ROC analysis showed that the AUC of serum IL-17,TGF-βand their combination detcetion was 0.819,0.825 and 0.920,respectively.Conclusion IL-17 and TGF-βin elderly patients with BP was significantly up-regulated,and are positively correlated with the severity of the disease.The combined detection of IL-17 and TGF-βis of high diagnostic value for BP in the elderly.

关 键 词：白细胞介素-17 转化生长因子-Β 细菌性肺炎 老年 肺泡灌洗液 

分 类 号：R563.1[医药卫生—呼吸系统]