银杏二萜内酯对急性tMCAO大鼠及慢性tMCAO大鼠的改善作用  

Improvement effect of diterpene ginkgolides on acute tMCAO rats and chronic tMCAO rats

作　　者：王玲 徐少锋 冯楠 王伟平 蔡杰 王晓良 WANG Ling;XU Shao-feng;FENG Nan;WANG Wei-ping;CAI Jie;WANG Xiao-liang(Institute of Materia Medica,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing Key Laboratory of New Drug Mechanisms and Pharmacological Evaluation Study(BZ0150),Beijing 100050,China)

机构地区：[1]中国医学科学院、北京协和医学院药物研究所,作用机制研究与药效评价北京市重点实验室(BZ0150),北京100050

出　　处：《药学学报》2023年第12期3669-3673,共5页Acta Pharmaceutica Sinica

基　　金：新药作用机制研究与药效评价北京市重点实验室(BZ0150)。

摘　　要：本文研究银杏二萜内酯(diterpene ginkgolides,DG)对短暂性大脑中动脉阻塞(transient middle cerebral artery occlusion,tMCAO)术后SD大鼠学习记忆的影响。利用线栓法造成大鼠tMCAO,主要分为假手术组、溶剂对照组、丁苯酞(NBP)组、DG组。本研究中的动物实验按照北京协和医学院福利伦理审查委员会的伦理指导方针进行(00000646、00000635)。利用神经行为学评分、脑梗塞体积测定、跳台和Morris水迷宫实验评价DG对tMCAO大鼠的改善作用。在急性tMCAO大鼠模型中:100mg·kg^(-1) DG能显著改善急性tMCAO模型的神经评分和梗死面积。在慢性tMCAO大鼠模型中,100mg·kg^(-1) DG组在生存率上比模型组有所提高,水迷宫学习期结果提示,与模型组比,100mg·kg^(-1) DG组具有改善的趋势,但弱于100mgkgNBP组;30mg·kg^(-1) DG给药组与模型组相比,无明显改善作用。综上所述,DG对急性tMCAO和慢性tMCAO均具有一定的治疗作用。To study the cognitive effects of diterpene ginkgolides(DG),transient middle cerebral artery occlusion(tMCAO)-induced rats were established.tMCAO-rats induced by suture method were divided into sham operation group,solvent control group,NBP group,DG group.The animal experiments in the present study were performed in accordance with the Ethical Guidelines of the Laboratory Animal Welfare Ethical Committee of Peking Union Medical College(00000646,00000635).The effects of DG on tMCAO rats were evaluated by neurological severity score,cerebral infarction volume measurement,step-down and Morris water maze test.In the acute tMCAO rat model,100 mg·kg^(-1) DG improved the neural score and infarction volume.In the chronic tMCAO rat model,DG 100 mg·kg^(-1) significantly improved the survival rate of tMCAO-induced rats.The Morris water maze results showed 100 mg·kg^(-1) DG decreased the latency of tMCAO-induced rats to find the platform,while the effect was weaker than the NBP.However,DG 30 mg·kg^(-1) did not show a significant effect.In conclusion,DG exerted a therapeutic effect on transient middle cerebral artery occlusion.

关 键 词：短暂性大脑中动脉阻塞 银杏二萜内酯 学习记忆 血管性痴呆 脑保护 

分 类 号：R966[医药卫生—药理学]