丝素蛋白自组装纳米粒的制备、理化表征及细胞相容性研究  

Preparation,physicochemical characterization and cytocompatibility study of self-assembled silk fibroin nanoparticles

作　　者：闫羽 程佳玲 刘子晗 王洪亮 杨艳芳 叶军 蒋伟哲[1] 刘玉玲 CHENG Jia-ing;YAN Yu;CHENG Jia-ling;LIU Zi-han;WANG Hong-liang;YANG Yan-fang;YE Jun;JIANG Wei-zhe;LIU Yu-ling(College of Pharmacy,Guangxi Medical University,Nanning 530021,China;Beijing Key Laboratory of Drug Delivery Technology and Novel Formulation,Institute of Materia Medica,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100050,China)

机构地区：[1]广西医科大学药学院,广西南宁530021 [2]中国医学科学院、北京协和医学院药物研究所,药物传输技术及新型制剂北京市重点实验室,北京100050

出　　处：《药学学报》2023年第12期3715-3721,共7页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目(82304393);北京市科技新星计划(Z211100002121127,20220484219);中国医学科学院医学与健康科技创新工程(2021-I2M-1-026)

摘　　要：本研究旨在制备丝素蛋白纳米粒(SF-NPs),并对制备得到的纳米粒的理化性质及细胞相容性进行评价。采用优化简易的去溶剂化法制备SF-NPs。通过单因素的处方筛选,如丝素蛋白(SF)溶液的浓度、SF溶液和有机溶剂的比例、超声功率及时间、不同有机相的种类,优化了制剂处方,并对最优处方的粒径分布、多分散性指数(PDI)、zeta电位、形态及稳定性进行了表征。通过CCK-8及细胞活/死染色Calcein-AM/PI评估了SF-NPs的体外细胞相容性。实验结果表明,当SF浓度为20 mg·mL^(-1)、水相和丙酮的体积比为1∶6、超声功率为80 W、超声时间为3 min时,制备得到的SF-NPs最优。本研究制备得到的SF-NPs为类球形,粒径分布狭窄,平均粒径为144.8 nm, PDI为0.174,zeta电位为-27.35 mV。细胞相容性实验结果表明, SF-NPs具有优异的细胞相容性,可促进细胞的增殖。综上表明,通过去溶剂法制备得到SF-NPs具有均一的粒径及良好的生物相容性,在药物递送领域具有较大的应用前景。This study aimed to prepare silk fibroin nanoparticles(SF-NPs)and assess the physicochemical properties and biocompatibility of the formulation.An optimized and simplified solvent displacement method was employed to obtain SF-NPs.Single-factor prescription screening,such as silk fibroin(SF)solution concentration,the ratio of SF solution to organic solvent,ultrasonication power and time,and different types of organic phases,was used to optimize the formulation.The characterization of the optimal formulation included particle size,polydispersity index(PDI),zeta potential,morphology,and stability.The in vitro cell compatibility of the nanoparticles was evaluated using CCK-8 and Calcein-AM/PI cell viability staining.The results showed that when SF concentration was 20 mg:mL^(-1),volume ratio of aqueous phase to acetone was 1:6,ultrasonic power was 80 W and ultrasonic time was 3 min,the best SF-NPs was obtained.The nanoparticles prepared in this study exhibit a near-spherical shape,with a uniform size distribution,having an average size of 144.8 nm,a PDI of 0.174,and a zeta potential of-27.35 mV.Results from in vitro cell experiments demonstrate excellent cell compatibility of SFNPs,showing the ability to promote cell proliferation.The SF-NPs which were successfully prepared in this study exhibit uniform particle size and excellent biocompatibility.

关 键 词：丝素蛋白 纳米粒 去溶剂法 处方筛选 细胞相容性 

分 类 号：R943[医药卫生—药剂学]