COVID-19确诊病例及灭活疫苗接种者不同时期血清IgA、IgM、IgG抗体检测结果比较  

Serum anti-SARS-CoV-2 IgA,IgM and IgG among confirmed COVID-19 cases at different stages and inactivated COVID-19 vaccine recipients after inoculation:a comparative analysis

作　　者：李东晓 卢世栋 胡晓 宋云 毋碧聪 李亚飞 李懿[1] 马红霞[1] 王海峰[1] 叶莹[1] 黄学勇[1] LI Dongxiao;LU Shidong;HU Xiao;SONG Yun;WU Bicong;LI Yafei;LI Yi;MA Hongxia;WANG Haifeng;YE Ying;HUANG Xueyong(Henan Provincial Center for Disease Control and Prevention,Zhengzhou 450016,China)

机构地区：[1]河南省疾病预防控制中心,郑州450016

出　　处：《中国公共卫生》2023年第9期1159-1164,共6页Chinese Journal of Public Health

基　　金：新发传染病监测与预警研究(211100311000);河南省中青年卫生健康科技创新领军人才培养项目(YXKC2020006);河南省疫情防控应急科研攻关项目(221111311300);重大传染病病原体监测、溯源及传播机制研究(221111311300)。

摘　　要：目的研究不同时期新型冠状病毒感染(COVID-19)病例及接种2019-nCoV灭活疫苗人群的抗体免疫应答规律及特点。方法于2020年1月—2021年10月采集河南省43例COVID-19确诊病例的86份血清标本,46名灭活疫苗接种者的184份血清标本,50名未感染健康人的50份血清标本,采用磁微粒化学发光法和酶联免疫吸附法对320份血清标本检测并分析其抗体水平。结果确诊病例恢复期IgA、IgM、IgG抗体阳性率高于急性期(P值均<0.05);恢复期的IgM、IgG抗体水平高于急性期(P值均<0.05)。灭活疫苗接种后31周,IgG和IgA抗体阳性率分别维持在63.04%(29/46)和36.96%(17/46),IgM抗体全部转阴;疫苗接种者第4、8、12、31周IgA抗体吸光度值差异无统计学意义(F=2.365,P=0.073),IgM、IgG抗体水平差异均有统计学意义(P<0.05)。同时期确诊病例的IgA、IgM抗体阳性率高于疫苗接种者,IgM、IgG抗体水平高于疫苗接种者(P值均<0.05)。结论IgG抗体在新型冠状病毒感染早期和恢复期阳性率最高。IgA、IgG抗体出现时间较IgM早,在疫苗接种者体内持续时间超过31周。同时期确诊病例的IgM、IgG抗体水平高于疫苗接种者。Objective To examine time-dependent variations in serum antibodies of severe acute respiratory disease coronavirus 2(SARS-CoV-2)among confirmed coronavirus disease 2019(COVID-19)cases at different stages and inactivated COVID-19 vaccine recipients after inoculation.Methods The study was conducted in a city of Henan province.Totally 86 serum samples were collected from 43 confirmed COVID-19 cases at acute stage(1th-23rd day of onset)and recovery state(25th-58th day of onset)during January 2020-October 2021;other 184 serum samples from 46 healthy recipients of inactivated COVID-19 vaccine at 4/8/12/31 weeks after inoculations and 50 serum samples from 50 residents without confirmed SARS-CoV-2 infections were also collected during March-October 2021.Anti-SARSCoV-2 immunoglobulin A/G/M(IgA/G/M)were detected with magnetic particle chemiluminescence method and enzyme-linked immunosorbent assay for all the serum samples.The levels of the serum antibodies among the three groups′participants were analyzed statistically.Results Among the confirmed COVID-19 cases,the positivity rates of anti-SARS-CoV-2 IgA,IgM and IgG of the serum samples collected at recovery stage were significantly higher than those at acute stage(all P<0.05);while,the concentrations of anti-SARS-CoV-2 IgM and IgG of the serum samples collected at recovery stage were significantly higher than those at acute stage(both P<0.05).Among the healthy COVID-19 vaccine recipients 31 weeks after the inoculations,the positivity rate was 63.04%(29/46)for anti-SARS-CoV-2 IgG and 36.96%(17/46)for anti-SARS-CoV-2 IgA,but all samples were negative for anti-SARS-CoV-2 IgM.No significant differences were observed in the absorbance ratios anti-SARS-CoV-2 IgA among the samples collected 4,8,12,and 31weeks after the inoculation(F=2.365,P=0.073),but there were significant differences in the concentrations of anti-SARS-CoV-2 IgM and IgG(both P<0.05)among the samples.Both the positivity rate of anti-SARS-CoV-2 IgA and IgM and the concentrations of anti-SARS-CoV-2 IgG and IgM

关 键 词：IGA抗体 IGG抗体 IgM抗体 血清学检测 COVID-19确诊病例 新冠病毒灭活疫苗接种者 

分 类 号：R563.1[医药卫生—呼吸系统] R186[医药卫生—内科学]