不同产地赤芝药材的质量分析  

Quality evaluation of Ganoderma lucidumfrom different areas

作　　者：曾秒 谢孟君 祝子喻 俞月婷 张梅[1] ZENG Miao;XIE Meng-jun;ZHU Zi-yu;YU Yue-ting;ZHANG Mei(State Key Laboratory of Southwestern Chinese Medicine Resources,School of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China)

机构地区：[1]成都中医药大学药学院西南特色中药资源国家重点实验室,四川成都611137

出　　处：《中草药》2023年第21期7193-7201,共9页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(81774202);成都中医药大学杏林学者学科人才科研提升计划(CXTD2018010)。

摘　　要：目的对不同产地批次赤芝Ganoderma lucidum进行全面的质量评价,为灵芝药材质量标准的完善及优质赤芝原料药的筛选提供依据。方法收集15批次各GAP基地的灵芝药材,参照《中国药典》2020年版方法及行业通用方法,考察其水分、灰分、水溶性浸出物、醇溶性浸出物,分别采用比色法、柱前衍生高效液相色谱法(PMP-HPLC)、高效凝胶渗透色谱法(HPGPC)测定多糖含量并进行比较;采用比色法测定三萜甾醇含量,HPLC法进行灵芝酸A一测多评;同时对15批赤芝的HPLC指纹图谱进行差异比较。结果15批赤芝药材均符合《中国药典》2020年版的质量标准要求,指纹图谱聚类分析和主成分分析(principal component analysis,PCA)结果表明山东聊城产赤芝能明显区别于其他批次样品。结论15批赤芝药材均符合《中国药典》质量标准,说明国内赤芝GAP基地种植质量良好;同时山东聊城产赤芝能显著区别于其他产地批次赤芝样品,主要体现在三萜甾醇等有效成分的含量更高。ObjectiveA comprehensive quality evaluation analysis of Chizhi[Ganodermalucidum(Leyss.exFr.)Karst.]samples from different batches ororigins was conducted to provide a basis for the improvement of the quality standard of Lingzhi(Ganoderma)and toscreeningof high-quality G.lucidummaterials.Methods Referring to the method of Chinese Pharmacopoeia 2020 editionand common approaches in this field,fifteen batches of G.lucidumherbs from each GAP base were collected and examined for moisture,ash,water-soluble leachate,alcohol soluble leachate,the polysaccharide content was compared by determining colorimetric method,precolumn derivatization high performance liquid chromatography(PMP-HPLC),and high performance gel permeation chromatography(HPGPC).The triterpene sterol content was determined by colorimetric method,and the QAMS of ganoderic acid A was evaluated by HPLC method..The fingerprint profiles of 15 batches of G.lucidumwere also compared for differences.Results All 15 batches of G.lucidummet the quality standard requirements of the Chinese Pharmacopoeia 2020 edition,the results of fingerprint cluster analysis and principal component analysis(PCA)show that G.lucidum of Liaocheng,Shandong Province can be clearly distinguished from other batches of G.lucidum.Conclusion All 15 batches of G.lucidummet the quality standards of theChinese Pharmacopoeia,indicating that the quality of the domestic G.lucidumGAP base cultivation is good;at the same time,G.lucidum of Liaocheng,Shandong Province can be significantly different from other origin batches of G.lucidumsamples,mainly in the higher content of active ingredients such as triterpene sterols.

关 键 词：灵芝 质量评价 主成分分析 多糖 三萜 指纹图谱 

分 类 号：R286.2[医药卫生—中药学]