B(A)血型的鉴定及临床输血探讨  

Identification and clinical transfusion of B(A)subgroup

作　　者：苗军[1] 耿明璐[1] 李建斌[1] 马晓莉[1] 杨贺才[1] 王丽萍 刘丹[1] 张珂 周川 程俊杰 MIAO Jun;GENG Minglu;LI Janbin;MA Xiaoli;YANG Hecai;WANG Liping;LIU Dan;ZHANG Ke;ZHOU Chuan;CHENG Junjie(Henan Red Cross Blood Center,Zhengzhou 450000,China;The Second Affiliated Hospital of Zhengzhou University;The Third Affiliated Hospital of Zhengzhou University)

机构地区：[1]河南省红十字血液中心,河南郑州450000 [2]郑州大学第二附属医院 [3]郑州大学第三附属医院

出　　处：《中国输血杂志》2023年第11期1012-1014,共3页Chinese Journal of Blood Transfusion

基　　金：河南省医学科技攻关联合共建项目(LHGJ20200251);河南省医学科技攻关项目(201503199)。

摘　　要：目的对B(A)血型进行血型血清学及分子生物学鉴定,分析探讨此类血型的合理输血方法。方法采用血清学和直接测序的方法对7例B(A)血型标本进行血清型和基因型检测,用盐水介质和抗人球蛋白卡交叉配血,分析7例标本输注红细胞的方案。结果7例标本的血型血清学结果相似,基因型中有3例为B(A)04/O01,2例为B(A)04/O02,2例为B(A)02/O01。7例B(A)血型标本与O型和B型献血者和标本主侧配血均为阴性。结论B(A)血型应结合基因分型技术正确定型,B(A)血型红细胞输血可选择O型和B型洗涤红细胞。Objective To carry out serological and molecular biological identification of B(A)subtype,and discuss the rational blood transfusion strategy.Methods Serological and direct sequencing methods were used to detect serotype and genotype of 7 cases of B(A)subtype,and cross matching was performed by saline medium and anti human globulin card to analyze the red blood cells(RBCs)transfusion strategy.Results The serology results of blood type of 7 samples were similar,with B(A)04/O01 in 3 cases,B(A)04/O02 in 2 cases and B(A)02/O01 in 2 cases.7 cases of B(A)subtypes were matched with randomly selected blood donors of type O and B on the major side.Conclusion B(A)subtypes should be identified by genotyping techniques.Washed RBCs of type B and O can be used for B(A)blood type transfusion.

关 键 词：ABO血型 B(A)血型 血型鉴定 交叉配血 

分 类 号：R457.11[医药卫生—治疗学]