基于“质-量”双标的侧柏叶质量分析方法研究  

Research on quality analysis method of Platycladi Cacumen based on“quality-quantity”double standard

作　　者：于浩 李天娇[1,2,3] 包永睿 王帅[1,2,3] 孟宪生 YU Hao;LI Tian-jiao;BAO Yong-rui;WANG Shuai;MENG Xian-sheng(College of Pharmacy,Liaoning University of Traditional Chinese Medicine,Dalian 116600,China;Liaoning Multi-dimensional Analysis of Traditional Chinese Medicine Technical Innovation Center,Dalian 116600,China;Liaoning Modern Traditional Chinese Medicine Research and Engineering Laboratory,Dalian 116600,China)

机构地区：[1]辽宁中医药大学药学院,辽宁大连116600 [2]辽宁省中药多维分析专业技术创新中心,辽宁大连116600 [3]辽宁省现代中药研究工程实验室,辽宁大连116600

出　　处：《中草药》2023年第22期7319-7324,共6页Chinese Traditional and Herbal Drugs

基　　金：辽宁省重点研发计划项目(2020JH2/10300088):基于中医典籍的经典名方槐花散药学研究。

摘　　要：目的建立以对照药材为基准物质的定性和不依赖多种对照品定量的侧柏Platycladus orientalis叶药材“质-量”双标控制方法。方法采用HPLC技术,以对照药材为基准物质建立侧柏叶特征图谱,通过Q-TOF-MS技术,鉴定共有峰的化学成分,以对照药材和供试药材特征峰的相似度,明确药材真伪,即“质”;对内标成分“柚皮苷”进行准确定量,以内标成分计,计算不同批次供试品中各特征峰的相对含量,取“平均数-标准差”作为特征峰化学成分相对于内标物质化学成分的相对含量下限,依据特征峰相对含量下限明确侧柏叶药材优劣,即“量”。结果建立的特征图谱及含量测定方法符合方法学考察要求;确定了11个共有色谱峰,鉴定出了4个化学成分,分别为杨梅苷、槲皮苷、穗花杉双黄酮、扁柏双黄酮,各侧柏叶供试药材与侧柏叶对照药材的相似度均大于0.90;规定了侧柏叶药材特征峰化学成分相对含量下限。结论该方法不依赖多种对照品,能清晰、快速地判断药材的真伪优劣,为侧柏叶的质量控制方法提供参考。Objective To establish a“quality-quantity”double standard control method for Cebaiye(Platycladi Cacumen),which is qualitative based on the reference traditional Chinese medicinal materials(TCMM)as standard substances and quantitative without relying on multiple reference substances.Methods HPLC technology was used to establish the characteristic chromatogram of Platycladi Cacumen based on the reference TCMM as standard substances.The chemical constituents of common peaks were identified by Q-TOF-MS technology,and the authenticity of Platycladi Cacumen was determined by the similarity of characteristic peaks of the reference TCMM and the tested TCMM.The above statement is“quality”.The relative content of each characteristic peak in different batches of the tested TCMM was calculated by accurate quantition of internal standard component naringin.The“average value-standard deviation”was taken as the lower limit of the relative content of characteristic peak chemical components relative to chemical composition of internal standard substance,and the quality of Platycladi Cacumen was determined according to the lower limit of the relative content of characteristic peak.The above statement is“quantity”.Results The characteristic chromatogram and content determination method met the requirements of methodology investigation.A total of 11 common chromatographic peaks were determined,and four chemical components were identified,which were myricetin,quercitrin,amentoflavone,hinokiflavone.The similarity between the tested TCMM and reference TCMM was greater than 0.90.The lower limit of relative content of chemical components of Platycladi Cacumen characteristic peak was defined.Conclusion The method can judge the authenticity of medicinal materials clearly and quickly without many reference substances,and provide reference for quality control of Platycladi Cacumen.

关 键 词：侧柏叶 对照药材 质量控制 特征图谱 杨梅苷 槲皮苷 穗花杉双黄酮 扁柏双黄酮 

分 类 号：R286.2[医药卫生—中药学]