常用消毒剂对噬菌体核酸污染的清除效果研究  

Effect of commonly used disinfectants on removal of bacteriophage nucleic acid contamination

作　　者：王慧敏 金慧[1] 陆烨[2] 曹阳 陈冰冰[1] 沈林海[1] WANG Hui-min;JIN Hui;LU Ye;CAO Yang;CHEN Bing-bing;SHEN Lin-hai(Hangzhou Center for Disease Control and Prevention,Hangzhou Zhejiang 310021;Zhejiang Provincial Center for Disease Control and Prevention,China)

机构地区：[1]杭州市疾病预防控制中心,浙江杭州310021 [2]浙江省疾病预防控制中心

出　　处：《中国消毒学杂志》2023年第11期805-806,809,共3页Chinese Journal of Disinfection

基　　金：浙江省医药卫生科技计划项目(2022KY129);杭州市农业与社会发展科研项目(20211231Y080)。

摘　　要：目的研究季铵盐消毒剂、75%乙醇、商品核酸清除剂和含氯消毒剂对MS2噬菌体核酸污染的清除效果。方法将1010 PFU;mL的噬菌体悬液滴加在无菌物体表面载体上,2000 mg;L双链季铵盐消毒剂、75%乙醇、核酸清除剂和有效氯1000、2000 mg;L含氯消毒剂作用10或30 min,荧光定量逆转录聚合酶链反应(RT-PCR)检测常用消毒剂对MS2噬菌体核酸的清除效果。结果作用30 min,含有效氯2000 mg;L的含氯消毒剂可完全清除MS2噬菌体基因,2000 mg;L双链季铵盐消毒剂、75%乙醇、商品核酸清除剂和有效氯1000 mg;L含氯消毒剂对MS2噬菌体基因清除效果较差。结论用有效氯2000 mg;L的含氯消毒剂可清除病毒核酸。Objective To investigate the removal effect of quaternary ammonium salt disinfectant,75%ethanol,commercial nucleic acid scavenger and chlorine-containing disinfectant on nucleic acid contamination of MS2 bacteriophages.Methods 1010 PFU;mL phage suspension droplets were added to the sterile surface carrier,and 2000 mg;L double-stranded quaternary ammonium salt disinfectant,75%ethanol,nucleic acid scavenger and 1000 and 2000 mg;L of available chlorine disinfectant were added to the sterile surface carrier for 10 or 30 min,and the removal effect of commonly used disinfectants on MS2 phage nucleic acid was detected by real-time reverse transcription polymerase chain reaction(RT-PCR).Results After 30 min,chlorine-containing disinfectant containing 2000 mg;L of available chlorine could completely remove the MS2 phage gene,and 2000 mg;L double-stranded quaternary ammonium disinfectant,75%ethanol,commercial nucleic acid scavenger and available chlorine 1000 mg;L chlorine-containing disinfectant had poor effect on the removal of MS2 phage gene.Conclusion Chlorine-containing disinfectant with available chlorine 2000 mg;L can be used to remove viral nucleic acids.

关 键 词：消毒剂 核酸污染 核酸清除 MS2噬菌体 

分 类 号：R187.2[医药卫生—流行病学]