Thirteen new peptaibols with antimicrobial activities from Trichoderma sp.  

作　　者：TANG Pan HUANG Dan ZHENG Kai-Xuan HU Dan DAI Ping LI Chuan-Hui QIN Sheng-Ying CHEN Guo-Dong YAO Xin-Sheng GAO Hao 

机构地区：[1]Institute of Traditional Chinese Medicine&Natural Products,College of Pharmacy/Guangdong Province Key Laboratory of Pharmacodynamic Constituents of TCM and New Drugs Research/International Cooperative Laboratory of Traditional Chinese Medicine Modernization and Innovative Drug Development of Ministry of Education(MOE)of China,Jinan University,Guangzhou 510632,China [2]Clinical Experimental Center,First Affiliated Hospital of Jinan University,Guangzhou 510630,China

出　　处：《Chinese Journal of Natural Medicines》2023年第11期868-880,共13页中国天然药物（英文版）

基　　金：supported by the National Key Research and Development Program of China(No.2018YFA0903200//2018YFA0903201);the National Natural Science Foundation of China(Nos.81925037 and 81973213);National High-level Personnel of Special Support Program(No.2017RA2259);the 111 Project of Ministry of Education of the People's Republic of China(No.B13038);Local Innovative and Research Teams Project of Guangdong Pearl River Talents Program(No.2017BT01Y036);Guangdong International Science and Technology Cooperation Base(No.2021A0505020015);Innovative and Research Teams Project of Guangdong Higher Education Institution(No.2021KCXTD001);K.C.Wong Education Foundation(No.Guo-Dong Chen,2021,China);the Science and Technology Planning Project of Guangzhou(No.202201020048)。

摘　　要：From the fungus Trichoderma sp., we isolated seven novel 18-residue peptaibols, neoatroviridins E-K ( 1−7 ), and six new 14-residue peptaibols, harzianins NPDG J-O ( 8−13 ). Additionally, four previously characterized 18-residue peptaibols neoatroviridins A-D ( 14−17 ) were also identified. The structural configurations of the newly identified peptaibols ( 1−13 ) were determined by comprehensive nuclear magnetic resonance (NMR) and high-resolution electrospray ionization tandem mass spectrometry (HR-ESI-MS/MS) data. Their absolute configurations were further determined using Marfey’s method. Notably, compounds 12 and 13 represent the first 14-residue peptaibols containing an acidic amino acid residue. In antimicrobial assessments, all 18-residue peptaibols ( 1 − 7 , 14 − 17 ) exhibited moderate inhibitory activities against Staphylococcus aureus 209P, with minimum inhibitory concentration (MIC) values ranging from 8−32 μg·mL^(−1). Moreover, compound 9 exhibited moderate inhibitory effect on Candida albicans FIM709, with a MIC value of 16 μg·mL^(−1).

关 键 词：PEPTAIBOLS Neoatroviridin Harzianin Trichoderma sp. Antibacterial activity 

分 类 号：R284[医药卫生—中药学]