地黄饮子对帕金森病肾虚证大鼠运动行为的影响  

Effects of Dihuang Yinzi on behavior function in Parkinson′s disease rats with kidney deficiency syndrome

作　　者：张世霞 李聚林[2] 李东明 路晓娟 闫川慧 Zhang Shixia;Li Julin;Li Dongming;Lu Xiaojuan;Yan Chuanhui(Basic Medical College,Shanxi University of Chinese Medicine,Jinzhong,Shanxi 030619,China;Heping Branch of Shanxi Traditional Chinese Medical Hospital,Taiyuan,Shanxi 030012,China;Hefei PreceDo pharmaceuticals Co.Ltd.,Hefei Anhui,230088,China)

机构地区：[1]山西中医药大学基础医学院,山西晋中030619 [2]山西省中医院和平分院,山西太原030012 [3]合肥中科普瑞生物医药科技有限公司,安徽合肥230088

出　　处：《中国药物与临床》2023年第5期273-277,共5页Chinese Remedies & Clinics

基　　金：国家自然科学基金(81803977)。

摘　　要：目的通过观察分析地黄饮子对帕金森病(PD)肾虚证大鼠模型实时步态行为的影响,探讨地黄饮子对PD肾虚证大鼠运动障碍的改善作用。方法按随机数字表法将90只大鼠随机分为空白对照组、模型组、阳性对照组、地黄饮子低剂量组、地黄饮子中剂量组、地黄饮子高剂量组6组。采用D-半乳糖+鱼藤酮建立大鼠PD肾虚证模型,阳性对照组给予25 mg/kg左旋多巴液,地黄饮子低、中、高剂量组分别给予5.36 g/kg、10.72 g/kg和21.45 g/kg地黄饮子,空白组和模型组大鼠给予等体积蒸馏水灌胃,药物干预3周后,对各组大鼠进行实时步态行为训练,训练结束后采集大鼠步态的时间、空间及距离参数并进行统计学分析。结果空白组的左、右侧布基分别为(85.7±1.4)mm,(86.7±2.3)mm,模型组大鼠左、右侧布基分别为(86.9±1.0)mm,(88.7±2.3)mm,与空白组比较,模型组左、右侧步基增大(P<0.05);低、中剂量组左侧布基分别为(85.6±0.7)mm,(85.7±1.8)mm,中、高剂量组及阳性对照组右侧布基分别为(86.5±1.4)mm,(86.5±2.3)mm,(86.9±2.9)mm,与模型组相比均显著降低(P<0.05);空白组右前足、右后足的步态周期分别为(333±143)s,(363±106)s,模型组右前足步、右后足态周期分别为(458±104)s,(495±121)s,均较空白组延长(P<0.05),中剂量组、阳性对照组右前足步态周期分别为(340±173)s,(338±119)s,右后足的步态周期分别为(378±126)s,(365±195)s,均较模型组明显缩短(P<0.05);足迹平均面积空白组左前足、右前足、右后足分别为(257±30)mm^(2),(231±8)mm^(2),(249±26)mm^(2),模型组分别为(262±14)mm^(2),(242±11)mm^(2),(266±12)mm^(2),均较空白组明显增加(P<0.05),中、高剂量组、阳性对照组左前足的足迹平均面积分别为(247±9)mm^(2),(260±6)mm^(2),(262±8)mm^(2),中剂量组、阳性对照组右前足的足迹平均面积分别为(233±9)mm^(2),(241±8)mm^(2),高剂量组右后足的足迹平均面积为(251±8)mm^(2),与模Objective To observe and analyze the effects of Dihuang Yinzi on real-time gait behavior of PD rats with kidney deficiency syndrome,and to explore the improvement effect of Dihuang Yinzi on its behavior impairment.Methods According to the random number table method,90 rats were randomly divided into 6 groups:blank control group,model group,positive control group,low dose group of Dihuang Yinzi,medium dose group of Dihuang Yinzi,and high dose group of Dihuang Yinzi.A rat model of PD kidney deficiency syndrome was established using D-galactose and rotenone.The rats in the positive control group were given 25 mg/kg of lev-odopa solution,while the rats in low,medium,and high dose groups of Dihuang Yinzi were gavaged with 5.36 g/kg,10.72 g/kg,and 21.45 g/kg,respectively.The rats in blank group and model group were gavaged with equal vol-ume of distilled water.After 3 weeks of drug intervention,real-time gait behavior training was conducted on rats of each group.After 3 weeks of drug intervention,the temporal,spatial and distance parameters of the rats′gait were collected at the end of the training and statistically analyzed.Results The left and right base ganglia of the blank group were(85.7±1.4)mm and(86.7±2.3)mm,respectively.The left and right basal ganglia of the model group rats were(86.9±1.0)mm and(88.7±2.3)mm,respectively.Compared with the blank group,the left and right basal ganglia of the model group increased(P<0.05).The left basal ganglia of the low and medium dose groups were(85.6±0.7)mm and(85.7±1.8)mm,respectively.The right basal ganglia of the medium,high dose group,and positive control group were(86.5±1.4)mm,(86.5±2.3)mm,and(86.9±2.9)mm,respectively,which were significant-ly reduced compared to the model group(P<0.05).The gait cycles of the blank group′s right anterior foot and right posterior foot were(333±143)s and(363±106)s,respectively.The gait cycles of the model group′s right anterior foot and right posterior foot were(458±104)s and(495±121)s,respectively,which were longer than

关 键 词：帕金森病 步态分析 地黄饮子 

分 类 号：R285.5[医药卫生—中药学]