粗毛纤孔菌子实体及其不同极性溶剂提取物止血活性  

Hemostatic Activity of Inonotus hispidus Fruiting Body Powder and Its Polar Solvent Extracts

作　　者：赵立智 包海鹰[1] 邹滨 王青春 韩晨 刘颖[1] ZHAO Lizhi;BAO Haiying;ZOU Bin;WANG Qingchun;HAN Chen;LIU Ying(College of Traditional Chinese Medicinal Materials,Jilin Agricultural University,Key Research Laboratory for the Development and Utilization of Fungi Traditional Chinese Medicine Resources,Changchun 130118,Jilin,China)

机构地区：[1]吉林农业大学中药材学院,菌类中药资源开发与利用重点研究室,吉林长春130118

出　　处：《食用菌学报》2023年第6期69-75,共7页Acta Edulis Fungi

基　　金：国家自然科学基金(32070021);吉农德百桑黄研究与产业开发中心(20210080)。

摘　　要：通过测定小鼠出血时间、凝血时间、凝血功能指标、血小板参数和红细胞参数,研究粗毛纤孔菌(Inonotus hispidus)子实体粉末组(高、低剂量分别为1.2、0.6 g·kg^(-1))、石油醚提取物组(高、低剂量分别为12、6 g·kg^(-1))、乙酸乙酯提取物组(高、低剂量分别为17、8.5 g·kg^(-1))、正丁醇提取物组(高、低剂量分别为45、22.5 g·kg^(-1))和水提取物组(高、低剂量分别为620、310 g·kg^(-1))对小鼠的止血活性。结果表明:与空白组相比,粗毛纤孔菌粉末,乙酸乙酯、水提取物高剂量组出血时间极显著减少;粉末高、低剂量组,石油醚、乙酸乙酯、水提取物高剂量组凝血时间显著减少;粉末高、低剂量组,石油醚、乙酸乙酯、正丁醇、水提取物高剂量组凝血酶原时间极显著减少;粉末高、低剂量组,乙酸乙酯、正丁醇、水提取物高剂量组活化部分凝血活酶时间显著减少;粉末、水提取物高、低剂量组,乙酸乙酯提取物高剂量组血浆凝血酶时间显著减少;粉末、石油醚提取物高剂量组纤维蛋白原含量显著降低;石油醚提取物高剂量组、水提取物低剂量组平均血小板体积显著增加;粉末、水提取物高、低剂量组血小板计数极显著升高;石油醚提取物高剂量组血小板分布宽度显著增加;粉末高、低剂量组,水提取物高剂量组血小板压积显著增加;石油醚提取物高剂量组红细胞数目极显著减少,血红蛋白含量极显著降低,平均红细胞体积极显著增加。研究结果将为利用粗毛纤孔菌的止血活性提供参考。By measuring bleeding time,coagulation time,coagulation function index,platelet parameters and red blood cell parameters of mice,different kinds of Inonotus hispidus fruiting body samples,including fruiting body powder(high dose 1.2 g·kg^(-1)and low dose 0.6 g·kg^(-1)),petroleum ether extract(high dose 12 g·kg^(-1)and low dose 6 g·kg^(-1)),ethyl acetate extract(high dose 17 g·kg^(-1)and low dose 8.5 g·kg^(-1)),n-butanol extract group(high dose 45 g·kg^(-1)and low dose 22.5 g·kg^(-1))and water extract(high dose 620 g·kg^(-1)and low dose 310 g·kg^(-1))were studied for hemostatic activity in mice.The results showed that high doses of I.hispidus powder,ethyl acetate extract and water extract extremely significantly reduced mouse bleeding time as compared with the blank control.Coagulation time was extremely significantly decreased in I.hispidus powder,high dose petroleum ether extract,high dose ethyl acetate extract,and high dose water extract groups as compared with that in the blank control.Prothrombin time was extremely significantly decreased in I.hispidus powder groups,high dose petroleum ether extract group,high dose ethyl acetate extract group,high dose n-butanol extract group and high dose water extract group.Activated partial thromboplastin time was significantly reduced in I.hispidus powder groups,high dose ethyl acetate extract group,high dose n-butanol extract group,and high dose water extract group.Plasma thrombin time was significantly reduced in I.hispidus powder groups,high dose ethyl acetate extract group,and water extract groups.High dose I.hispidus powder and high dose petroleum ether extract significantly reduced fibrinogen content.High dose petroleum ether extract and the low dose water extract resulted in a significant increase in mean platelet volume.High dose petroleum ether extract also significantly increased platelet distribution width and average red blood cell volume.Platelet count was significantly increased in I.hispidus powder and water extract groups.Plateletcrit was signifi

关 键 词：粗毛纤孔菌 止血 凝血 

分 类 号：R285.5[医药卫生—中药学]