杨树桑黄水提液对果蝇寿命和繁殖力及寿命相关基因的影响  

Effect of Sanghuangporus vaninii Water Extract on Life Span,Fecundity and Life Span-Related Genes of Drosophila melanogaster

作　　者：侯立娟[1] 卯明娟 刘迪[2] 蒋宁[1] 周蓉云 HOU Lijuan;MAO Mingjuan;LIU Di;JIANG Ning;ZHOU rongyun(Institute of Vegetable Crops,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,Jiangsu,China;Yanbian University,Yanji 136200,Jilin,China)

机构地区：[1]江苏省农业科学院蔬菜研究所,江苏南京210014 [2]延边大学农学院,吉林延吉136200

出　　处：《食用菌学报》2023年第6期86-91,共6页Acta Edulis Fungi

基　　金：江苏省农业科技自主创新项目[CX(22)3141];国家食用菌产业技术体系(CARS-20);常州市科技支撑计划(CE20212005)。

摘　　要：研究不同质量浓度(0.1、1.0、10.0、50.0μg·mL^(-1))杨树桑黄(Sanghuangporus vaninii)水提液对果蝇平均寿命、半致死时间、最长寿命、蛹数量和F1代羽化成虫数量的影响;采用定量PCR方法检测1μg·mL^(-1)杨树桑黄水提液饲喂果蝇后第1、3、5、10、20天与寿命、免疫相关基因的表达量。结果表明:在实验范围内,与对照相比,1.0μg·mL^(-1)水提液延长果蝇平均寿命,0.1、1.0μg·mL^(-1)水提液延长成虫半致死时间;1.0μg·mL^(-1)水提液增加F1代羽化成虫数量;基因TEP、BrC, E74B、JHEH均在第3天相对表达量较高;基因INDY、EcRB、Srl、TPI均在第10天相对表达量较高;基因TotA,、Dros、TotX均在第20天相对表达量较高。The effects of different concentrations(0.1, 1, 10, 50 μg· mL^(-1)) of Sanghuangporus vaninii water extract on average life span, median-lethal time, maximum life span, number of pupae and number of F1 adults of Drosophila melanogaster were studied. The expression levels of 13 genes pertaining to longevity, immunity and energy metabolism were determined by qPCR at 1, 3, 5, 10 and 20 d upon feeding D. melanogaster with 1 μg· mL^(-1) S. vaninii water extract. The results showed that in the experimental range, 1.0 μg·mL^(-1) water extract prolonged median-lethal time, average life span, and increasesd F1 adult number as compared with the control.Median-lethal time was also prolonged under 0.1 μg· mL^(-1) S. vaninii water extract. The relative expression levels of TEP, BrC, E74B and JHEH were higher at 3 d than other time. The expression levels of INDY, EcRB,Srl and TPI were higher at 10 d than other time. The expression levels of TotA, Dros and TotX were higher at 20 d than other time.

关 键 词：桑黄 果蝇 寿命 药用功能 基因表达 

分 类 号：R285.5[医药卫生—中药学]