羊毛角蛋白融合型丝素蛋白膜材料的性能分析  

Performance analysis of fibroin membrane materials mixed with wool keratin

作　　者：常雪宁 何海波 王依辰 曾頔 代方银[1,2,3] 赵天福[1,2,3] CHANG Xuening;HE Haibo;WANG Yichen;ZENG Di;DAI Fangyin;ZHAO Tianfu(State Key Laboratory of Resource Insects,Southwest University,Chongqing 400715,China;College of Sericulture,Textile and Biomass Science,Southwest University,Chongqing 400715,China;Chongqing Engineering Research Center of Biomaterial Fiber and Modern Textile,Southwest University,Chongqing 400715,China)

机构地区：[1]西南大学资源昆虫高效养殖与利用全国重点实验室,重庆400715 [2]西南大学蚕桑纺织与生物质科学学院,重庆400715 [3]西南大学重庆市生物质纤维材料与现代纺织工程技术研究中心,重庆400715

出　　处：《丝绸》2023年第12期28-35,共8页Journal of Silk

基　　金：国家现代农业产业技术体系项目(CARS-18-ZJ102);家蚕分子育种法创制种质资源的快速纯系化技术探究项目(20220412204853557)。

摘　　要：羊毛角蛋白关联蛋白(KAPs)是一类富含半胱氨酸的纤维蛋白,可以氧化形成二硫键,与疏水相互作用和范德华力一起赋予角蛋白高的机械强度和结构稳定性。为了扩展天然蚕丝蛋白质材料的研究,本文以通过转基因法得到的KAP融入型蚕丝为材料,制备丝素蛋白膜,检测分析了膜的结构和性能。发现转KAP基因丝素膜含有更多的β-折叠结构,KAP的融入可以显著提高膜的机械性能和疏水性,融入不同种类的KAP可以获得性能不同的融合蛋白材料。通过转基因法制取蚕丝/羊毛角蛋白混合蛋白膜具有方法简便、无污染等特点,可为天然蚕丝材料的研究和应用提供新的思路。Silk has been used in China for thousands of years and is the only natural filament fiber in nature that can be mass-produced,which is of great significance in the textile industry.In recent years,protein films,a derivative material of silk,have attracted much attention and are widely used in the fields of antimicrobial dressings,bone repair scaffolds,slow-release carriers and biosensors,and have become a high-tech material with a promising future.Despite the superior mechanical properties of native silk,the strength and toughness of regenerated silk-protein membranes are unsatisfactory,which greatly hinders their wide application.To enhance the mechanical strength of regenerated sericin materials,some scholars have enhanced the mechanical strength of regenerated sericin materials by mixing natural proteins,such as wool keratin and spider silk protein,and also improved the softness and ductility of regenerated sericin materials by adding non-protein components,such as glycerol and polyvinyl alcohol,of which glycerol can also promote the interactions between the molecules of sericin and the water molecules to improve the hydrophilicity of sericin.Both protein and non-protein addition methods are often accompanied by problems such as complex dissolution,cumbersome process,waste of resources and environmental pollution.Wool keratin-associated proteins(KAPs)are fibrous proteins rich in cysteine,capable of forming disulphide bonds upon oxidation.These bonds,along with hydrophobic interactions and van der Waals forces,contribute to the high mechanical strength and structural stability of keratin.In this study,KAP-incorporated silk was generated through transgenic methods and used as the material.Silk protein membranes were then obtained by gradually removing water from the silk protein solution and shaping it accordingly.Furthermore,1%glycerol was added to the silk solution to modify the protein membrane.We tested the secondary structure,physicochemical properties,and mechanical properties of ordinary sericin protein m

关 键 词：丝素 羊毛角蛋白 再生蛋白膜 结构 机械性能 亲水性 

分 类 号：TS102.3[轻工技术与工程—纺织工程]