植物病原菌对解偶联剂的抗性机制研究进展  

Research progress on the resistance mechanism of plant pathogens to uncouplers

作　　者：程星凯 张俊婷 刘鹏飞 刘西莉 CHENG Xingkai;ZHANG Junting;LIU Pengfei;LIU Xili(College of Plant Protection,China Agricultural University,Beijing 100193,China)

机构地区：[1]中国农业大学植物保护学院,北京100193

出　　处：《农药学学报》2023年第6期1198-1205,共8页Chinese Journal of Pesticide Science

基　　金：国家重点研发计划项目(2022YFD1400900)。

摘　　要：以氟啶胺为代表的解偶联剂具有低毒、广谱和高效的特点,对病原真菌、卵菌和细菌均表现出良好的抑菌活性。然而随着杀菌剂频繁而大量的使用,有害生物发展出越来越严重的抗药性。开展病原菌对杀菌剂的抗性机制研究,能够有效预防或治理病原菌抗药性。病原菌对杀菌剂的抗性机制解析方法通常以杀菌剂的靶标蛋白为线索展开,但由于氟啶胺这类杀菌剂在病原菌体内可能不是通过与靶标蛋白结合而产生的抑菌作用,使得通过寻找抗性突变体中发生变化的氨基酸位点,进而进行抗性机制解析的方法难以奏效。本综述以氟啶胺和我国自主创制的杀菌剂双苯菌胺为研究对象,对其作用机制及病原菌对其抗性机制的研究进展进行归纳总结,旨在为这类杀菌剂的田间科学使用提供参考,同时可为病原菌多药抗性机制的解析提供借鉴,丰富杀菌剂抗性研究体系,并能够在实践中为病原菌的抗性治理提供依据。Uncouplers represented by fluazinam show low toxicity,broad-spectrum,and high-efficiency activity,and exhibit strong virulence against pathogenic fungi,oomycetes,and bacteria.However,pathogens have developed increasingly severe resistance to fungicides due to their frequent and extensive use.Investigating the resistance mechanism of plant pathogens to fungicides will contribute to effective delaying or managing resistance.The analysis method for the resistance mechanism of fungicides is usually based on the target protein,but this does not apply to uncouplers as their antifungal efficacy may not occur through target protein binding in pathogens.It is unable to seek the changed amino acid sites in the resistant mutants,making it extremely difficult for exploring resistance mechanisms to uncouplers.This review focuses on the fluazinam and self-developed fungicide SYP-14288 in China and summarizes the research progress of their mode of action and resistance mechanism.The study could provide reference both for the scientific use of uncouplers in the field and for the disclosure of multidrug resistance mechanism,therefore enriching the research system of fungicide resistance and providing a basis for resistance management in practice.

关 键 词：病原菌 解偶联剂 抗性机制 代谢抗性 外排抗性 抗性治理 

分 类 号：S481.4[农业科学—农药学]