木质素基苯醚甲环唑纳米颗粒构建及防控杨梅凋萎病研究  

Construction of lignin-based difenoconazole nanoparticles and control of bayberry twig blight disease

作　　者：张启 张家栋 方云[1] 熊秋雨 王嵘 程敬丽[1] 孙鹂[3] 赵金浩[1] ZHANG Qi;ZHANG Jiadong;FANG Yun;XIONG Qiuyu;WANG Rong;CHENG Jingli;SUN Li;ZHAO Jinhao(Ministry of Agriculture and Rural Affairs Key Lab of Molecular Biology of Crop Pathogens and Insects,Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province,Zhejiang University,Hangzhou 310058,China;Lanxi Economic and Speciality Products Technology Promotion Centre,Lanxi 321100,Zhejiang Province,China;Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China)

机构地区：[1]浙江大学农业农村部作物病虫分子生物学重点实验室,浙江省作物病虫生物学重点实验室,杭州310058 [2]浙江省兰溪市经济特产技术推广中心,浙江兰溪321100 [3]浙江省农业科学院,杭州310021

出　　处：《农药学学报》2023年第6期1312-1321,共10页Chinese Journal of Pesticide Science

基　　金：浙江省“三农九方”科技协作项目(2022SNJF031);浙江省公益技术应用研究-分析测试项目(LGC22C140001)。

摘　　要：杨梅凋萎病传染性强,发病快,给杨梅生产带来了巨大损失。木质素基材料因来源广泛、价格便宜、且活性官能团较多,已广泛应用于纳米药物递送领域。为寻求具有缓释功能的药剂,以便更好地防控杨梅凋萎病,以苯醚甲环唑(difenoconazole,以下简称Di)为供试药剂,用苯甲酸酐对木质素磺酸钠(LS)进行疏水性改性后,负载Di制备了纳米颗粒Di@BLS,通过核磁共振氢谱(1H NMR)、傅里叶红外光谱(FT-IR)、扫描电子显微镜(SEM)及动态光散射激光粒度仪(DLS)等对其结构进行了表征,通过Turbiscan稳定性分析仪(TSI)对样品稳定性进行了分析;采用QuEChERs方法提取叶片中的Di,研究Di@BLS在杨梅中的吸收转运情况;最后对收集的杨梅病枝进行致病菌的分离鉴定,并分别采用菌丝生长速率法和盆栽试验法研究了Di@BLS对杨梅凋萎病菌的抑制效果。结果表明:在BLS载体浓度为1%、料药比为5:1、质量浓度为0.2%的SDS用量条件下制备的Di@BLS平均粒径为135.2 nm,该配方在大量减少表面活性剂用量的同时,可以保持与苯醚甲环唑微乳剂(Di ME)相近的制剂稳定性;吸收转运和田间试验均证实Di@BLS能延缓Di的降解,比Di ME在杨梅体内持留时间更久;菌丝生长速率法测定结果显示,Di@BLS对杨梅凋萎病菌可可毛色二孢菌Lasiodiplodia的EC50值为0.643μg/mL,与Di ME的接近;盆栽试验结果表明,在200μg/mL质量浓度下,相比Di ME,Di@BLS可降低杨梅凋萎病发病率2.3%。研究结果表明,所制备的纳米颗粒Di@BLS在杨梅体内具有较长的持效期和较好的抑菌效果,可为杨梅凋萎病的防控提供理论依据。Twig blight disease has the characteristics of strong infectivity and rapid onset,which has brought great losses to the production of bayberry fruit.Lignin-based materials are widely used in the field of nanomedicine delivery due to their wide source,low price,and more active functional groups.In order to seek agents with slow-release function for better control of twig blight disease,Di@BLS nanoparticles were prepared by hydrophobically modifying sodium lignosulfonate(LS)and loading difenoconazole(hereafter referred to as Di)as a test agent,which was characterized by 1H nuclear magnetic resonance(1H NMR),fourier transform infrared spectroscopy(FT-IR),scanning electron microscopy(SEM),dynamic light scattering laser particle sizing(DLS),and the stability of the samples was analyzed by turbiscan stability analyzer(TSI);Di in leaves was extracted by QuEChERs method,and the uptake and translocation of Di@BLS in bayberry were investigated,and finally,diseased branches of bayberry collected for the isolation and identification of pathogenic fungi were carried out.The inhibitory effect of Di@BLS on the bayberry twig blight disease was investigated using the mycelial growth rate method and the pot experiment,respectively.The results showed that the average particle size of Di@BLS was 135.2 nm at a BLS carrier concentration of 1%,a feed/drug ratio of 5:1 and a SDS dosage of 0.2%by mass.The optimized formulation could maintain stability similar to that of Di ME while reducing the amount of surfactant.Both absorption transfer and field experiments confirmed that Di@BLS could delay drug degradation and persist longer in bayberry than Di ME.The results of the mycelial growth rate method showed that the EC50 value of Di@BLS against Lasiodiplodia was 0.643μg/mL,which is close to that of Di ME.In the pot experiment,at the mass concentration of 200μg/mL,Di@BLS could reduce the incidence of blight of bayberry by 2.3%compared with Di ME.The results showed that the prepared nanoparticles Di@BLS had a long duration and good antifun

关 键 词：木质素磺酸钠 疏水性改性 苯醚甲环唑 纳米颗粒 杨梅凋萎病 防治效果 

分 类 号：TQ450.68[化学工程—农药化工]