氟虫腈/β-环糊精衍生物/壳聚糖纳米微球的制备及性能  

Preparation and properties of fipronil/β-cyclodextrin derivatives/chitosan nanoparticles

作　　者：张红丽 张仙娜 赵雅宁 玄光善[1] ZHANG Hongli;ZHANG Xianna;ZHAO Yaning;XUAN Guangshan(College of Chemical Engineering,Qingdao University of Science and Technology,Qingdao 266042,Shandong Province,China)

机构地区：[1]青岛科技大学化工学院,山东青岛266042

出　　处：《农药学学报》2023年第6期1322-1332,共11页Chinese Journal of Pesticide Science

摘　　要：本研究首先将β-环糊精(β-CD)进行修饰,以水难溶性氟虫腈(FP)为模型农药,制备了以羧甲基-β-环糊精(CM-β-CD)和羧甲基-羟丙基-β-环糊精(CM-HP-β-CD)为载体的FP包合物(ICs),然后ICs与壳聚糖(CS)通过静电相互作用形成纳米微球(NPs),并对纳米微球进行了表征。同时考察了包合物的溶解度、纳米微球的储存稳定性及光稳定性、体外释放行为。结果表明,FP经CM-β-CD和CM-HP-β-CD作用后可形成1:1的包合物,在水中的溶解度分别提高了102.34和76.29倍。载药纳米微球外观呈规则球形,粒径分布在200 nm左右,包封率最高可达90.42%。紫外光照射48 h后,2种纳米微球药物降解率均小于30%,明显低于FP原药的降解率(88.8%)。从释放曲线得知纳米微球中的FP具有缓释性能,且释放符合KorsmeyerPeppas模型,并在偏碱性条件下具有更高的释放率,这有助于提高对消化液偏碱性虫类的杀虫效果。In this study,β-cyclodextrin(β-CD)was modified and fipronil(FP)was used as a model pesticide,the inclusion complex(ICs)of carboxymethyl-β-cyclodextrin(CM-β-CD)and carboxymethyl-hydroxypropyl-β-cyclodextrin(CM-HP-β-CD)was prepared,then ICS and chitosan(CS)were interacted with each other to form nanoparticles(NPs)via electrostatic interaction,and the NPS were characterized.At the same time,the solubility of inclusion complex,the storage stability and light stability of nano-microspheres,and the release behavior in vitro were investigated.The results showed that FP formed 1:1 inclusion complexes with CM-β-CD and CM-HP-β-CD,increasing its solubility in water by 102.34 and 76.29 times,respectively.The drug-loaded nano-microspheres have a regular spherical appearance,the particle size distribution is about 200 nm,and the encapsulation efficiency can reach up to 90.42%.After UV irradiation for 48 h,the degradation rate of both nanoparticles was less than 30%,which was significantly lower than that of FP(88.8%).The release profiles showed the NPs provided sustained release of FP,following the Korsmeyer-Peppas model.The NPs also exhibited a higher release rate under alkaline conditions,which could help improve insecticidal efficacy of alkaline insects in the digestive juice.

关 键 词：氟虫腈 Β-环糊精衍生物 壳聚糖 纳米微球 缓释性能 

分 类 号：TQ450.68[化学工程—农药化工]