Transcriptome-based analysis of key genes and pathways affecting the linoleic acid content in chickens  

作　　者：ZHAO Wen-juan YUAN Xiao-ya XIANG Hai MA Zheng CUI Huan-xian LI Hua ZHAO Gui-ping 

机构地区：[1]Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding,Foshan University,Foshan 528000,P.R.China [2]Institute of Animal Science,Chinese Academy of Agricultural Sciences,Beijing 100193,P.R.China

出　　处：《Journal of Integrative Agriculture》2023年第12期3744-3754,共11页农业科学学报（英文版）

基　　金：This study was supported by the China Agriculture Research System of MOF and MARA(CARS-41);the Key-Area Research and Development Program of Guangdong Province,China(2020B020222002);the Foshan University High-level Talent Program,China(CGZ07243);the Guangdong Provincial Key Laboratory of Animal Molecular Design and Precise Breeding,China(2019B030301010);the Key Laboratory of Animal Molecular Design and Precise Breeding of Guangdong Higher Education Institutes,China(2019KSYS011);the Foshan Institute of Science and Technology Postgraduate Free Exploration Fund,China(2021ZYTS36).

摘　　要：Linoleic acid is an essential polyunsaturated fatty acid that cannot be synthesized by humans or animals themselves and can only be obtained externally.The amount of linoleic acid present has an impact on the quality and flavour of meat and indirectly affects consumer preference.However,the molecular mechanisms influencing the deposition of linoleic acid in organisms are not clear.As the molecular mechanisms of linoleic acid deposition are not well understood,to investigate the main effector genes affecting the linoleic acid content,this study aimed to screen for hub genes in slow-type yellow-feathered chickens by transcriptome sequencing(RNA-Seq)and weighted gene coexpression network analysis(WGCNA).We screened for candidate genes associated with the linoleic acid content in slow-type yellow-feathered broilers.A total of 399 Tiannong partridge chickens were slaughtered at 126 days of age,fatty acid levels were measured in pectoral muscle,and pectoral muscle tissue was collected for transcriptome sequencing.Transcriptome sequencing results were combined with phenotypes for WGCNA to screen for candidate genes.KEGG enrichment analysis was also performed on the genes that were significantly enriched in the modules with the highest correlation.A total of 13310 genes were identified after quality control of transcriptomic data from 399 pectoral muscle tissues.WGCNA was performed,and a total of 26 modules were obtained,eight of which were highly correlated with the linoleic acid content.Four key genes,namely,MDH2,ATP5B,RPL7A and PDGFRA,were screened according to the criteria|GS|>0.2 and|MM|>0.8.The functional enrichment results showed that the genes within the target modules were mainly enriched in metabolic pathways.In this study,a large-sample-size transcriptome analysis revealed that metabolic pathways play an important role in the regulation of the linoleic acid content in Tiannong partridge chickens,and MDH2,ATP5B,RPL7A and PDGFRA were screened as important candidate genes affecting the linoleic acid content.The r

关 键 词：CHICKEN linoleic acid transcriptome sequencing weighted gene coexpression network analysis(WGCNA) metabolic pathways 

分 类 号：S831[农业科学—畜牧学]