Characteristics of Mycobacterium tuberculosis serine protease Rv1043c in enzymology and pathogenicity in mice  

作　　者：TANG Yang-yang CUI Ying-ying JIANG Yan-yan SHAO Ming-zhu ZANG Xin-xin DANG Guang-hui LIU Si-guo 

机构地区：[1]State Key Laboratory for Animal Disease Control and Prevention,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150069,P.R.China

出　　处：《Journal of Integrative Agriculture》2023年第12期3755-3768,共14页农业科学学报（英文版）

基　　金：This research was supported by the National Key Research and Development Program of China(2021YFD1800403);the National Natural Science Foundation of China(32273005 and 32002256).

摘　　要：The serine proteases of Mycobacteria tuberculosis(Mtb)are important contributors to the process of bacterial invasion and its pathogenesis.In the present study,we systematically characterized the role of the Rv1043c protein in Mycobacterium infection by purifying the Rv1043c protein in Escherichia coli and constructing a Mycobacterium smegmatis(Msg)strain overexpressing Rv1043c(Msg_Rv1043c).We found that Rv1043c had serine protease activity and localized to the surface of Mtb.We determined that the optimal pH and temperature for the Rv1043c serine protease were 9.0 and 45°C,respectively.Moreover,the serine protease activity of Rv1043c was enhanced by divalent metal ions of Ca^(2+)and Mg^(2+).Site-directed mutagenesis studies demonstrated that the serine 279 residue in Rv1043c plays a catalytic role.Additionally,mouse model studies confirmed that Rv1043c significantly enhanced the survival of Msg in vivo,induced pulmonary injury and lung cell apoptosis,and promoted the release of pro-inflammatory cytokines interleukin-1βand interleukin-6 in mice.This study presents novel insights into the relationship between mycobacterial serine protease and the pathogenesis of the disease.

关 键 词：Mycobacterium tuberculosis Mycobacterium smegmatis serine protease Rv1043c PATHOGENICITY 

分 类 号：S855.2[农业科学—临床兽医学]