Biotechnology ofα-linolenic acid in oilseed rape(Brassica napus)using FAD2 and FAD3 from chia(Salvia hispanica)  

作　　者：XUE Yu-fei Inkabanga Tseke ALAIN YIN Neng-wen JIANG Jia-yi ZHAO Yan-ping LU Kun LI Jia-na DING Yan-song ZHANG Shi-qing CHAI You-rong 

机构地区：[1]Integrative Science Center of Germplasm Creation in Western China(Chongqing)Science City and Southwest University/Chongqing Key Laboratory of Crop Quality Improvement/College of Agronomy and Biotechnology,Southwest University,Chongqing 400715,P.R.China [2]Engineering Research Center of South Upland Agriculture,Ministry of Education/Academy of Agricultural Sciences,Southwest University,Chongqing 400715,P.R.China [3]Faculty of Agricultural Sciences,National Pedagogic University(UPN),Kinshasa 8815,D.R.Congo

出　　处：《Journal of Integrative Agriculture》2023年第12期3810-3815,共6页农业科学学报（英文版）

基　　金：This work was supported by the National Natural Science Foundation of China(31871549,32001441 and 32272015);the Chongqing Research Program of Basic Research and Frontier Technology,China(cstc2015jcyjBX0143);the Fundamental Research Funds for the Central Universities,China(XDJK2020C038);the National Key R&D Program of China(2016YFD0100506);the Young Eagles Program of Chongqing Municipal Commission of Education,China(CY220219)。

摘　　要：α-Linolenic acid(ALA,18:3Δ9,12,15)is an essential fatty acid for humans since it is the precursor for the biosynthesis of omega-3 long-chain polyunsaturated fatty acids(LC-PUFA).Modern people generally suffer from deficiency of ALA because most staple food oils are low or lack ALA content.Biotechnological enrichment of ALA in staple oil crops is a promising strategy.Chia(Salvia hispanica)has the highest ALA content in its seed oil among known oil crops.In this study,the FAD2 and FAD3 genes from chia were engineered into a staple oil crop,oilseed rape(Brassica napus),via Agrobaterium tumefaciens-mediated transformation of their LP4-2A fusion gene construct driven by the seed-specific promoter P_(NapA).In seeds of T0,T1,and T2 lines,the average ALA contents were 20.86,23.54,and 24.92%,respectively,which were 2.21,2.68,and 3.03 folds of the non-transformed controls(9.42,8.78,and 8.22%),respectively.The highest seed ALA levels of T0,T1,and T2 plants were 38.41,35.98,and 39.19%respectively,which were 4.10-4.77 folds of the respective controls.FA-pathway enzyme genes(BnACCD,BnFATA,BnSAD,BnSCD,BnDGAT1,BnDGAT2,and BnDGAT3)and positive regulatory genes(BnWRI1,BnLEC1,BnL1L,BnLEC2,BnABI3,BnbZIP67,and BnMYB96)were all significantly up-regulated.In contrast,BnTT1,BnTT2,BnTT8,BnTT16,BnTTG1,and BnTTG2,encoding negative oil accumulation regulators but positive secondary metabolism regulators,were all significantly down-regulated.This means the foreign ShFAD2-ShFAD3 fusion gene,directly and indirectly,remodeled both positive and negative loci of the whole FA-related network in transgenic B.napus seeds.

关 键 词：BIOTECHNOLOGY α-linolenic acid oilseed rape(Brassica napus) FAD2 FAD3 chia(Salvia hispanica) 

分 类 号：S565.4[农业科学—作物学]